Pedersen K E, Klitgaard N A, Jest P
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Scand J Clin Lab Invest. 1987 Dec;47(8):801-11.
New methods for determining the volume and sodium content of human mononucleated cells are presented and the kinetics of sodium efflux are characterized. Sodium efflux obeyed first-order kinetics with a half-life of about 9 min. The ouabain-sensitive sodium pump is responsible for about 60% of the total turnover of this cation. Reliable measurement of cell volume and sodium content requires pre-incubation at physiological conditions. Determination of sodium efflux should be based on the initial (less than 15 min) cellular delay of 22Na-activity in preloaded cells. In normal subjects (n = 34), mean cell volume was 635 +/- 138 nl/10(6) cells and sodium content averaged 14.6 +/- 2.8 mmol/l cell. Total and ouabain-sensitive efflux rate constants were 4.56 +/- 0.66 h-1 and 2.70 +/- 0.47 h-1. Corresponding absolute sodium efflux averaged 65.1 +/- 14.8 and 38.9 +/- 9.6 mmol/l/h, respectively.
本文介绍了测定人单核细胞体积和钠含量的新方法，并对钠外流动力学进行了表征。钠外流遵循一级动力学，半衰期约为9分钟。哇巴因敏感的钠泵约占该阳离子总周转率的60%。可靠测量细胞体积和钠含量需要在生理条件下进行预孵育。钠外流的测定应基于预加载细胞中22Na活性的初始（小于15分钟）细胞延迟。在正常受试者（n = 34）中，平均细胞体积为635 +/- 138 nl/10(6)细胞，钠含量平均为14.6 +/- 2.8 mmol/l细胞。总外流速率常数和哇巴因敏感外流速率常数分别为4.56 +/- 0.66 h-1和2.70 +/- 0.47 h-1。相应的绝对钠外流平均分别为65.1 +/- 14.8和38.9 +/- 9.6 mmol/l/h。
