Lang R, Adler I D
Mutat Res. 1977;48(1):75-88. doi: 10.1016/0027-5107(77)90191-9.
A dominant-lethal test and a heritable translocation test were performed with methyl methanesulphonate (MMS) at 40 mg/kg by treating the sensitive periods of post-meiotic spermatogenesis i.e. spermatozoa and spermatids. In the dominant-lethal test 25 to 60% dominant-lethal mutations were obtained depending on the mating intervals. In the heritable translocation test 11% sterile and partially sterile F1 males were observed in 250 offspring of the MMS group. All of the 14 partially sterile and 6 of the 14 sterile F1 males were demonstrated to be translocation carriers. Fertility of the partial steriles was about 40% of normal fertility. The translocation frequencies in the primary spermatocytes of the partially sterile F1 males varied between 2 and 99%. Transmission of partial sterility and translocations was confirmed in the F2 generation. There were no partially sterile or sterile males among the 245 controls.
通过在减数分裂后精子发生的敏感期（即精子和精细胞）用40毫克/千克的甲磺酸甲酯（MMS）进行处理，开展了显性致死试验和可遗传易位试验。在显性致死试验中，根据交配间隔获得了25%至60%的显性致死突变。在可遗传易位试验中，在MMS组的250只后代中观察到11%的F1雄性不育和部分不育。14只部分不育的F1雄性中的全部以及14只不育的F1雄性中的6只被证明是易位携带者。部分不育者的生育力约为正常生育力的40%。部分不育的F1雄性初级精母细胞中的易位频率在2%至99%之间变化。在F2代中证实了部分不育和易位的传递。245只对照中没有部分不育或不育的雄性。
