Shimada A, Egami N
Mutat Res. 1984 Feb;125(2):221-7. doi: 10.1016/0027-5107(84)90072-1.
Males of the fish Oryzias latipes were treated with various chemicals and then mated with normal females. The fertility and hatchability of the eggs laid by the parents were examined, and the dominant lethal effects were estimated. Mitomycin C induced dominant lethals in the fish spermatids and spermatocytes after the males had been treated with concentrations of 2.5 and 25 micrograms/ml. Methyl methanesulfonate (MMS) induced dominant lethals in spermatozoa and spermatozoa and spermatids after the injection of 200 and 400 mg/kg. These results are in good agreement with the results obtained with mice. However, the effects of ethyl methanesulfonate (EMS) were not clear on spermatogenic cells at any stage. We could not recognize any significant induction of dominant lethals by urethanes, bleomycin, caffeine, and two kinds of food-color additives, at least under the present experimental conditions.
用各种化学物质处理青鳉雄鱼，然后使其与正常雌鱼交配。检查亲本所产卵的受精率和孵化率，并估计显性致死效应。在用浓度为2.5微克/毫升和25微克/毫升的丝裂霉素C处理雄鱼后，它会在鱼的精子细胞和精母细胞中诱导显性致死。注射200毫克/千克和400毫克/千克的甲基磺酸甲酯（MMS）后，会在精子、精子细胞和精细胞中诱导显性致死。这些结果与用小鼠获得的结果非常一致。然而，在任何阶段，甲磺酸乙酯（EMS）对生精细胞的影响都不明确。至少在目前的实验条件下，我们未发现聚氨酯、博来霉素、咖啡因和两种食用色素添加剂有任何显著的显性致死诱导作用。
