Sugimura T, Eissner G
Natl Inst Anim Health Q (Tokyo). 1976 Winter;16(4):152-9.
Typing of foot-and-mouth disease (FMD) virus was performed by the direct fluorescent antibody (FA) technique. Type-specific FA was prepared from the following two sorts of procedures: (1) FA against live virus (FA-live) was prepared from hyperimmune serum taken from guinea pigs having received live FMD virus. Then it was adsorbed with concentrated heterotype antigen. (2) FA against inactivated virus (FA-Inact) was prepared from antiserum taken from guinea pigs immunized with purified FMD virus inactivated with acetylethyleneimine. Seventeen strains of FMD virus (seven strains of type A, seven strains of type O, and three strains of thpe C) were used. Type-specific FMD virus antigen was detected distinctly from the monolayer of BHK cells infected with each type of virus and fixed in acetone, in spite of negative results obtained from the cells fixed in methyl alcohol. All the 17 strains were typed successfully by the implementation of these two FA methods.
口蹄疫（FMD）病毒分型采用直接荧光抗体（FA）技术进行。型特异性FA通过以下两种方法制备：（1）抗活病毒FA（FA-活）由接种过口蹄疫活病毒的豚鼠的超免疫血清制备。然后用浓缩的异型抗原进行吸附。（2）抗灭活病毒FA（FA-灭活）由用乙酰亚胺灭活的纯化口蹄疫病毒免疫的豚鼠的抗血清制备。使用了17株口蹄疫病毒（7株A型、7株O型和3株C型）。尽管用甲醇固定的细胞结果为阴性，但从感染每种病毒并经丙酮固定的BHK细胞单层中能清晰检测到型特异性口蹄疫病毒抗原。通过实施这两种FA方法，成功对所有17株病毒进行了分型。
