一种基于简单磁盘法，利用硼酸化合物检测肺炎克雷伯菌碳青霉烯酶型β-内酰胺酶的方法。
Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type beta-lactamase by use of a boronic acid compound.
作者信息
Doi Yohei, Potoski Brian A, Adams-Haduch Jennifer M, Sidjabat Hanna E, Pasculle Anthony W, Paterson David L
机构信息
Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA.
出版信息
J Clin Microbiol. 2008 Dec;46(12):4083-6. doi: 10.1128/JCM.01408-08. Epub 2008 Oct 15.
Abstract
A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type beta-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type beta-lactamases from those producing other types of beta-lactamases.
摘要
评估了一种以碳青霉烯类为底物、3-氨基苯硼酸为抑制剂的纸片增效法用于检测肺炎克雷伯菌碳青霉烯酶（KPC）型β-内酰胺酶。当与对厄他培南不敏感相结合时，该方法易于操作，并且能够可靠地将产KPC型β-内酰胺酶的菌株与产其他类型β-内酰胺酶的菌株区分开来。
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