Huang Hui, Zheng Xiao Lin, Zheng Jun Song, Pan Jing, Pu Xiao Yun
Department of Clinical Laboratory Science, Third Military Medical University, Chongqing, 400038, China.
Biomed Microdevices. 2009 Feb;11(1):213-6. doi: 10.1007/s10544-008-9226-z.
A rapid and sensitive chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads for determination of alpha-fetoprotein (AFP) is described. In this system we use CO(2) laser to fabricate microfluidic chip, and use super-paramagnetic microbeads as solid carrier of antibody, chemiluminescence as detection signal. With this system we can perform AFP analysis within 20 min, and the linear range of AFP concentration is 1 approximately 800 ng/mL, the detection limit is 0.23 ng/mL. By this method no separation or preconcentration steps are needed. Most of all, the chip can be reused.
描述了一种基于超顺磁性微珠的快速灵敏的化学发光微流免疫分析系统，用于测定甲胎蛋白（AFP）。在该系统中，我们使用二氧化碳激光制造微流芯片，并使用超顺磁性微珠作为抗体的固体载体，以化学发光作为检测信号。利用该系统，我们可以在20分钟内完成AFP分析，AFP浓度的线性范围为1至800 ng/mL，检测限为0.23 ng/mL。通过这种方法，无需分离或预浓缩步骤。最重要的是，芯片可以重复使用。
