Roy Bishnupada, Dasgupta Shyamashree, Tandon Veena
Department of Zoology, North-Eastern Hill University, Shillong 793 022, India.
Microsc Res Tech. 2009 Feb;72(2):61-6. doi: 10.1002/jemt.20643.
The ultrastructural alterations in the tegument of Fasciolopsis buski in response to incubation in the alcoholic extract of Alpinia nigra were evaluated using transmission electron microscopy. The body tegument of the trematode is composed of an external syncytial layer, musculature, and an inner layer containing tegumental cells. The syncytium comprises various organelles like mitochondria, lysosomes, and tegumentary bodies of the type 2 kind with rare sighting of the type 1. Severe distortion and disorganization of the tegument was revealed in the parasite exposed to the A. nigra extract in the current study. The extent of vacuolization was such that vacuoles proceeded down to the basal lamina causing the syncytium to separate from the tegument at different places. There was depletion of parenchyma material and loss of connecting tubules running down from the syncytium to the tegumental cells causing the cells to be deprived of any proper boundaries.
利用透射电子显微镜评估了布氏姜片吸虫体表在黑豆蔻醇提取物中孵育后的超微结构变化。吸虫的体表由外部的合胞体层、肌肉组织以及包含皮层细胞的内层组成。合胞体包含各种细胞器，如线粒体、溶酶体和2型皮层小体，1型则少见。在本研究中，暴露于黑豆蔻提取物的寄生虫体表出现了严重的变形和紊乱。空泡化程度如此之高，以至于空泡一直延伸到基膜，导致合胞体在不同部位与体表分离。实质物质减少，从合胞体延伸到皮层细胞的连接小管丧失，导致细胞失去任何正常边界。
