Department of Zoology, North-Eastern Hill University, Shillong, India.
Vet Parasitol. 2010 Apr 19;169(1-2):214-8. doi: 10.1016/j.vetpar.2009.12.024. Epub 2010 Jan 4.
The alcoholic extract of Lysimachia ramosa Wall (Primulaceae) was tested in vitro against helminth parasites, Fasciolopsis buski and Ascaris suum, from porcine hosts and Raillietina echinobothrida from domestic fowl. The live adult parasites, collected from a freshly autopsied host, were exposed to different concentrations (5-50mg) of the test plant extract in physiological phosphate-buffered saline (PBS) having 0.1% dimethyl sulphoxide (DMSO) at 37+/-1 degrees C. The treated parasites revealed complete inactivation and flaccid paralysis that was followed by death at varying periods of time. A dose-dependent loss of motility and mortality was observed in all the treated parasites. Scanning electron microscopic observations revealed conspicuous deformity of the surface architecture in all the parasites exposed to the test plant extract. The general tegument in F. buski showed shrinkage and loss of scale-like spines; proglottides all along the strobilar length in R. echinobothrida appeared shrunken and deformed and the cuticular surface of A. suum appeared disorganised, having lost transverse striations. The botanicals of the test plant seem to be effective against all the three types of helminth parasites.
金丝桃属植物（报春花科）的醇提物在体外测试时，对来自猪宿主的片形吸虫和猪蛔虫，以及来自家禽的瑞利绦虫均具有活性。从新鲜剖检的宿主中收集的活成虫寄生虫，在 37±1°C 的含有 0.1％二甲亚砜（DMSO）的生理磷酸盐缓冲盐水（PBS）中，暴露于不同浓度（5-50mg）的测试植物提取物中。处理后的寄生虫完全失活并呈现松弛性麻痹，随后在不同时间死亡。所有接受治疗的寄生虫都表现出运动能力的剂量依赖性丧失和死亡率。扫描电子显微镜观察显示，所有暴露于测试植物提取物的寄生虫的表面结构都明显变形。片形吸虫的一般外皮显示出收缩和失去鳞片状刺；瑞利绦虫的整个虫体都出现了收缩和变形，猪蛔虫的外皮表面看起来混乱，失去了横向条纹。该植物提取物的植物似乎对所有三种类型的寄生虫都有效。
