Reddy Thulasimala, Gibbs Gerard M, Merriner D Jo, Kerr Jeffrey B, O'Bryan Moira K
Monash Institute of Medical Research, Monash University, Melbourne, Australia.
Dev Dyn. 2008 Nov;237(11):3313-23. doi: 10.1002/dvdy.21738.
The Cysteine-RIch Secretory Proteins (CRISPs) are abundantly produced in the male reproductive tract of mammals and within the venom of reptiles and have been shown to regulate ion channel activity. CRISPs, along with the Antigen-5 proteins and the Pathogenesis related-1 (Pr-1) proteins, form the CAP superfamily of proteins. Analyses of EST expression databases are increasingly suggesting that mammalian CRISPs are expressed more widely than in the reproductive tract. We, therefore, conducted a reverse transcription PCR expression profile and immunohistochemical analyses of 16 mouse tissues to define the sites of production of each of the four murine CRISPs. These data showed that each of the CRISPs have distinct and sometimes overlapping expression profiles, typically associated with the male and female reproductive tract, the secretory epithelia of exocrine glands, and immune tissues including the spleen and thymus. These investigations raise the potential for a role for CRISPs in general mammalian physiology.
富含半胱氨酸的分泌蛋白（CRISPs）在哺乳动物的雄性生殖道以及爬行动物的毒液中大量产生，并且已被证明可调节离子通道活性。CRISPs与抗原5蛋白和病程相关蛋白1（Pr-1）一起，构成了CAP蛋白超家族。对EST表达数据库的分析越来越多地表明，哺乳动物CRISPs的表达比在生殖道中更为广泛。因此，我们对16种小鼠组织进行了逆转录PCR表达谱分析和免疫组织化学分析，以确定四种小鼠CRISPs各自的产生部位。这些数据表明，每种CRISPs都有独特的、有时重叠的表达谱，通常与雄性和雌性生殖道、外分泌腺的分泌上皮以及包括脾脏和胸腺在内的免疫组织有关。这些研究增加了CRISPs在一般哺乳动物生理学中发挥作用的可能性。
