Kaparullina E N, Fedorov D N, Doronina N V, Trotsenko Iu A
Prikl Biokhim Mikrobiol. 2008 Jul-Aug;44(4):399-403.
A system of primers was designed on the basis of analysis of nucleotide sequences of the emoA gene encoding ethylenediaminetetraacetate (EDTA) monooxygenase, which are deposited in GenBank. This system of primers makes it possible to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. Polymerase chain reaction (PCR) of total DNA isolated from enrichment and pure cultures showed that this system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria. Partial sequences of emoA genes of bacteria of the genera Chelativorans and Stenotrophomonas, which are able to degrade this pollutant, have been sequenced and deposited in GenBank.
基于对编码乙二胺四乙酸（EDTA）单加氧酶的emoA基因核苷酸序列的分析设计了一套引物，这些序列保存在GenBank中。该引物系统能够从EDTA细菌分解剂中获得长度约为750 bp的emoA基因片段。从富集培养物和纯培养物中分离的总DNA进行的聚合酶链反应（PCR）表明，该系统可有效地用于检测α-和γ-变形杆菌中的emoA基因。已对能够降解这种污染物的Chelativorans属和嗜麦芽窄食单胞菌属细菌的emoA基因部分序列进行了测序并保存在GenBank中。
