Spiridonova E M, Berg I A, Kolganova T V, Ivanovskiĭ R N, Kuznetsov B B, Turova T P
Mikrobiologiia. 2004 May-Jun;73(3):377-87.
Based on the analysis of GenBank nucleotide sequences of the cbbL and cbbM genes, coding for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC), the key enzyme of the Calvin cycle, a primer system was designed that allows about 800-bp-long fragments of these genes to be PCR-ampliflied in various photo- and chemotrophic bacteria. The efficiency of the designed primer system in detection of RuBPC genes was demonstrated in PCR with DNA of taxonomically diverse bacteria possessing RuBPC genes with a known primary structure. Nucleotide sequences of RuBPC gene fragments of bacteria belonging to the genera Acidithiobacillus. Ectothiorhodospira, Magnetospirillum, Methylocapsa, Thioalkalispira, Rhodobacter, and Rhodospirillum were determined to be deposited with GenBank and to be translated into amino acid sequences and subjected to phylogenetic analysis.
基于对编码卡尔文循环关键酶核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBPC）大亚基的cbbL和cbbM基因的GenBank核苷酸序列分析，设计了一种引物系统，可在各种光合细菌和化能营养细菌中通过PCR扩增出这些基因约800 bp长的片段。在对具有已知一级结构的RuBPC基因的分类学上不同的细菌DNA进行PCR时，证明了所设计引物系统检测RuBPC基因的效率。测定了嗜酸氧化硫硫杆菌属、外硫红螺菌属、嗜磁螺菌属、甲基球菌属、嗜碱硫螺旋菌属、红杆菌属和红螺菌属细菌的RuBPC基因片段的核苷酸序列，存入GenBank，并翻译成氨基酸序列，进行系统发育分析。
