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使用微量样本通过冰点降低法评估渗透压测量。

An evaluation of osmolality measurement by freezing point depression using micro-amounts of sample.

作者信息

Koumantakis G, Wyndham L E

机构信息

Biochemistry Department Royal North Shore Hospital of Sydney Sydney 2065 Australia.

出版信息

J Automat Chem. 1989;11(2):80-3. doi: 10.1155/S1463924689000167.

DOI:10.1155/S1463924689000167
PMID:18925239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2547790/
Abstract

An evaluation of the Advanced micro-osmometer is presented. This instrument has been shown to have an excellent analytical precision (within-run CV = 0.59%, between-day CV = 0.58%). It is accurate over an analytical range of 0-2000 mmol/kg of osmolality shown by linearity studies and split sample correlations against vapour pressure osmometry, freezing point osmometry and an external quality assurance programme. Analytical errors due to operator technique are almost eliminated because of good instrument design. Preliminary results on whole-blood osmolality are included. The required sample size of 20 mul permits osmolality measurements on most clinical samples. It is concluded that the Advanced micro-osmometer satisfies laboratory requirements.

摘要

本文介绍了对高级微量渗透压计的评估。该仪器已被证明具有出色的分析精度(批内变异系数CV = 0.59%,日间变异系数CV = 0.58%)。通过线性研究以及与蒸气压渗透压计、冰点渗透压计和外部质量保证计划的分割样本相关性表明,它在0 - 2000 mmol/kg渗透压的分析范围内是准确的。由于仪器设计良好,几乎消除了因操作人员技术导致的分析误差。文中还包括了全血渗透压的初步结果。所需的20微升样本量允许对大多数临床样本进行渗透压测量。结论是高级微量渗透压计满足实验室要求。

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