Cordero Chiara, Bicchi Carlo, Galli Mario, Galli Stefano, Rubiolo Patrizia
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Torino, Italy.
J Sep Sci. 2008 Oct;31(19):3437-50. doi: 10.1002/jssc.200800280.
A series of OV1-OV1701 column sets, in which the two dimensions differ in id and/or film thickness, were adopted to separate components of the volatile and semivolatile fraction of samples of plant origin. In particular two applications are presented: a target analysis approach to determine volatile suspected allergens in a medium-complexity fragrance and in sandalwood essential oil, and a fingerprint analysis approach to compare herbal extracts. Some basic chromatographic parameters of these combinations, i. e. net separation measure (S(GC x GC)), degree of orthogonality and a suitable number of modulations per peak, were also estimated by analysing two test mixtures: FAME (C4:0-C24:0) and suspected volatile allergens. Experimental results show that 0.25 mm homologous id column combination, despite their lower separation power, have good capacity to separate and resolve medium-to-complex fractions from samples of plant origin, demonstrating: (i) good system orthogonality, maximized through proper exploitation of stationary-phase selectivity and (ii) reduced 2-D column overloading effects due to the increased 2-D mass loadability, thus facilitating the analysis of mixtures whose components differ significantly in relative abundance.
采用一系列OV1 - OV1701柱组，其中二维在内径和/或膜厚方面有所不同，以分离植物源样品中挥发性和半挥发性部分的成分。特别介绍了两种应用：一种目标分析方法，用于测定中等复杂度香料和檀香精油中的挥发性疑似过敏原；另一种指纹分析方法，用于比较草药提取物。通过分析两种测试混合物：脂肪酸甲酯（C4:0 - C24:0）和疑似挥发性过敏原，还估算了这些组合的一些基本色谱参数，即净分离度（S(GC x GC)）、正交度和每个峰合适的调制次数。实验结果表明，0.25 mm同系物内径柱组合尽管分离能力较低，但具有良好的分离和解析植物源样品中中等至复杂馏分的能力，表现为：（i）良好的系统正交性，通过适当利用固定相选择性实现最大化；（ii）由于二维质量负载能力增加，二维柱过载效应降低，从而便于分析其成分相对丰度差异显著的混合物。
