Sundov Zeljko, Tomić Snjezana, Vilović Katarina, Kunac Nenad, Kalebić Marija, Bezić Josko
Department of Internal Medicine, Split University School of Medicine, Split, Croatia.
Croat Med J. 2008 Oct;49(5):636-42. doi: 10.3325/cmj.2008.5.636.
To demonstrate immunohistochemical expression of matrix metalloproteinase-2 (MMP-2) protein in Duke's B colon cancer and determine its correlation with age, sex, grade, presence of vascular invasion, and patients' overall survival.
The study took place from January 1995 to December 1997. We determined the expression of MMP-2 in 152 formalin-fixed, paraffin embedded specimens of Duke's B colon carcinomas by immunohistochemical analysis using MMP-2 monoclonal antibody. Immunohistochemical expression was scored semiquantitatively. Carcinomas were graded as low or high grade. Survival time was analyzed with Kaplan-Meier method, and the log-rank test was used to assess the differences between groups. Cox proportional hazard regression model was used for multivariate survival analysis.
Univariate analysis showed that positive staining for MMP-2, high histological grade, vascular invasion, male sex, and age>60 years were associated with shorter survival in patients with Duke's B colon cancer (P range from 0.023 to <0.001). Multivariate analysis showed that only MMP-2 overexpression (P<0.001; hazard ratio [HR]=3.64) and vascular invasion (P<0.001; HR=4.27) were associated with shorter overall survival.
Expression of MMP-2 is an important independent indicator of shorter survival in patients with Duke's B colon cancer and should be taken into consideration in decision-making on the use of adjuvant systemic therapy in patients with Duke's B colon cancer.
证实基质金属蛋白酶-2（MMP-2）蛋白在杜克B期结肠癌中的免疫组化表达，并确定其与年龄、性别、分级、血管侵犯情况及患者总生存期的相关性。
研究于1995年1月至1997年12月进行。我们使用MMP-2单克隆抗体通过免疫组化分析确定了152例福尔马林固定、石蜡包埋的杜克B期结肠癌标本中MMP-2的表达。免疫组化表达进行半定量评分。癌组织分为低级别或高级别。生存时间采用Kaplan-Meier法分析，对数秩检验用于评估组间差异。Cox比例风险回归模型用于多因素生存分析。
单因素分析显示，MMP-2阳性染色、高组织学分级、血管侵犯、男性及年龄>60岁与杜克B期结肠癌患者生存期较短相关（P值范围为0.023至<0.001）。多因素分析显示，只有MMP-2过表达（P<0.001；风险比[HR]=3.64）和血管侵犯（P<0.001；HR=4.27）与总生存期较短相关。
MMP-2的表达是杜克B期结肠癌患者生存期较短的一个重要独立指标，在决定对杜克B期结肠癌患者使用辅助全身治疗时应予以考虑。
