De Cock K M, Porter A, Kouadio J, Maran M, Lafontaine M F, Gershy-Damet G M, Heyward W L, George R
Projet RETRO-Cl, Abidjan, Côte d'Ivoire, Africa.
AIDS. 1991 Jul;5(7):859-63. doi: 10.1097/00002030-199107000-00010.
To examine cross-reactivity of antibodies to heterologous antigens, on HIV-1 and HIV-2 Western blots, we tested sera from 1362 consecutive tuberculosis (TB) patients and 2127 consecutive blood donors. Specimens positive on enzyme-linked immunosorbent assay (ELISA) for HIV-1 or HIV-2 were further characterized by synthetic peptide-based tests, and tested by HIV-1- and HIV-2-specific Western blots. Dual serologic reactivity on synthetic peptide tests was proportionately more frequent in HIV-positive TB patients than in blood donors, and HIV-2 reactivity less frequent. Positive HIV-1 Western blots were seen in 73-83% of specimens specifically characterized as positive for HIV-2 on synthetic peptide tests. Cross-reactivity to HIV-2 Western blots by HIV-1-positive specimens was significantly more frequent in TB patients (35%) than in asymptomatic donors (9%; P less than 0.001). Using recently recommended criteria for HIV-2 Western blot interpretation (presence of two env bands) reduced the overall proportion of HIV-1-positive specimens having a positive HIV-2 Western blot from 27.5 to 16.4%, with [corrected] minimal effect on sensitivity in the diagnosis of HIV-2 reactivity on specimens positive on synthetic peptide tests.
为检测抗体对异源抗原的交叉反应性，我们在HIV-1和HIV-2免疫印迹试验中，检测了1362例连续性肺结核（TB）患者和2127例连续性献血者的血清。酶联免疫吸附测定（ELISA）检测HIV-1或HIV-2呈阳性的标本，通过基于合成肽的试验进一步鉴定，并进行HIV-1和HIV-2特异性免疫印迹试验。在HIV阳性的TB患者中，合成肽试验的双重血清学反应比献血者中更常见，而HIV-2反应则较少见。在合成肽试验中被特异性鉴定为HIV-2阳性的标本中，73%-83%出现HIV-1免疫印迹阳性。HIV-1阳性标本对HIV-2免疫印迹的交叉反应在TB患者中（35%）显著高于无症状献血者（9%；P<0.001）。采用最近推荐的HIV-2免疫印迹解释标准（出现两条env带），使HIV-1阳性标本中HIV-2免疫印迹阳性的总体比例从27.5%降至16.4%，对合成肽试验阳性标本中HIV-2反应性诊断的敏感性影响[校正后]最小。
