Ferrari P, Trabaud M A, Rommain M, Mandine E, Zalisz R, Desgranges C, Smets P
Laboratories Cassenne, Centre de Recherches Immunologiques Roussel-Uclaf, Cergy Pontoise, France.
AIDS. 1991 Jul;5(7):865-70. doi: 10.1097/00002030-199107000-00011.
Trichosanthin was purified from fresh Chinese root tubers of Trichosanthes kirilowii and evaluated for anti-HIV activity. Trichosanthin inhibited syncytium formation between infected H9 cells and uninfected Sup-T1 cells from 0.5 to 4 micrograms/ml. Trichosanthin also inhibited HIV replication in H9 and CEM-SS cells at 1 microgram/ml, but was toxic for MT-4 cells (HTLV-I-positive), at doses greater than 0.25 microgram/ml. This new purification procedure confirms the anti-HIV activity of trichosanthin on some cell lines in different biological assays.
天花粉蛋白从新鲜的栝楼中国根块茎中纯化得到，并对其抗HIV活性进行了评估。天花粉蛋白在0.5至4微克/毫升的浓度下抑制受感染的H9细胞与未感染的Sup-T1细胞之间的合胞体形成。天花粉蛋白在1微克/毫升的浓度下也抑制H9和CEM-SS细胞中的HIV复制，但在剂量大于0.25微克/毫升时对MT-4细胞（HTLV-I阳性）有毒性。这种新的纯化方法在不同的生物学试验中证实了天花粉蛋白对某些细胞系的抗HIV活性。
