Yeung H W, Li W W
Int J Pept Protein Res. 1987 Mar;29(3):289-92. doi: 10.1111/j.1399-3011.1987.tb02256.x.
beta-Trichosanthin was a new abortifacient protein purified from the Chinese drug, Wangua, root tubers of Trichosanthes cucumeroides (Cucurbitaceae). The purification procedure involved acetone fractionation, ammonium sulfate precipitation, ion-exchange chromatography on CM-Sepharose and preparative agarose electrophoresis. Homogeneity of beta-trichosanthin was demonstrated in immunoelectrophoresis, agarose electrophoresis and SDS-polyacrylamide gel electrophoresis. It had a molecular weight of 28,000 and no cysteine in its molecule. It differed from trichosanthin, a known abortifacient protein isolated from a related Chinese drug, Tianhuafen, root tubers of Trichosanthes kirilowii (Cucurbitaceae), in molecular weight, carbohydrate content, charge and amino acid composition. beta-Trichosanthin was, however, immunochemically identical to trichosanthin and was about twice as potent as trichosanthin in inducing mid-term abortion in mice.
β-天花粉蛋白是从中药瓜蒌（葫芦科栝楼属植物双边栝楼的块根）中纯化得到的一种新型堕胎蛋白。纯化过程包括丙酮分级分离、硫酸铵沉淀、CM-琼脂糖离子交换色谱和制备性琼脂糖电泳。β-天花粉蛋白的同质性在免疫电泳、琼脂糖电泳和SDS-聚丙烯酰胺凝胶电泳中得到证实。它的分子量为28000，分子中不含半胱氨酸。它与从相关中药天花粉（葫芦科栝楼属植物栝楼的块根）中分离得到的已知堕胎蛋白天花粉蛋白在分子量、碳水化合物含量、电荷和氨基酸组成方面有所不同。然而，β-天花粉蛋白在免疫化学上与天花粉蛋白相同，并且在诱导小鼠中期流产方面的效力约为天花粉蛋白的两倍。
