Ly Elodie, Piot Olivier, Durlach Anne, Bernard Philippe, Manfait Michel
Unité MéDIAN UMR CNRS 6237 MEDyC, UFR de Pharmacie, IFR 53, Université Reims-Champagne Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France.
Appl Spectrosc. 2008 Oct;62(10):1088-94. doi: 10.1366/000370208786049187.
Polarized Raman microspectroscopy can provide precious information regarding the orientation and ordering of the molecules in a sample without staining or particular preparation. This technique is used for the first time on a human skin section to probe the molecular modifications of the surrounding dermis in superficial basal cell carcinoma. Spectra using polarized and conventional Raman microspectroscopies were recorded on dermis bordering either the tumor or healthy epidermis. Band areas and spectral decomposition on selected vibrations were computed. Significant differences in dermal collagen vibration bands are detected using both polarized and conventional micro-spectroscopies, but the spectral changes between tumor and healthy tissues are enhanced using polarized Raman microspectroscopy. The analysis of these spectral differences highlights structural modifications of the triple helix of collagen. We see polarized Raman microspectroscopy as a potential tool that could be implemented for clinical analyses to guide clinicians and surgeons in the treatment of aggressive skin cancers. The information obtainable could also help better elucidate the molecular mechanisms induced in basal cell carcinoma development.
偏振拉曼显微光谱术无需染色或特殊制备，就能提供有关样品中分子取向和排列的宝贵信息。该技术首次应用于人体皮肤切片，以探测浅表基底细胞癌周围真皮的分子变化。使用偏振拉曼显微光谱术和传统拉曼显微光谱术记录了肿瘤或健康表皮周围真皮的光谱。计算了选定振动的谱带面积和光谱分解。使用偏振和传统显微光谱术均检测到真皮胶原振动谱带的显著差异，但偏振拉曼显微光谱术增强了肿瘤组织与健康组织之间的光谱变化。对这些光谱差异的分析突出了胶原三螺旋的结构变化。我们认为偏振拉曼显微光谱术是一种潜在的工具，可用于临床分析，以指导临床医生和外科医生治疗侵袭性皮肤癌。所获得的信息也有助于更好地阐明基底细胞癌发生过程中诱导的分子机制。
