恢复一条代谢途径。

Restoring a metabolic pathway.

作者信息

Richard John P

机构信息

Department of Chemistry, University at Buffalo, SUNY, Buffalo, New York 14260-3000, USA.

出版信息

ACS Chem Biol. 2008 Oct 17;3(10):605-7. doi: 10.1021/cb800238s.

DOI:10.1021/cb800238s

PMID:18928248

Abstract

Gluconeogenesis is blocked in a strain of Escherichia coli that is deficient in triosephosphate isomerase, but it was restored by the insertion of a plasmid coding for an L-glyceraldehyde 3-phosphate reductase (YghZ). This reductase provides a "bypass" that produces dihydroxyacetone phosphate (DHAP) by the consecutive enzyme-catalyzed reduction of L-glyceraldehyde 3-phosphate ( L-GAP) by NADPH to give L-glycerol 3-phosphate and reoxidation by NAD(+) catalyzed by endogenous L-glycerol 3-phosphate dehydrogenase to give DHAP. The origin of cellular L-GAP remains to be determined.

摘要

在一株缺乏磷酸丙糖异构酶的大肠杆菌中，糖异生作用受阻，但通过插入编码L-甘油醛3-磷酸还原酶（YghZ）的质粒得以恢复。这种还原酶提供了一条“旁路”，通过NADPH连续酶促还原L-甘油醛3-磷酸（L-GAP）生成L-甘油3-磷酸，再由内源性L-甘油3-磷酸脱氢酶催化被NAD(+)重新氧化生成磷酸二羟丙酮（DHAP）。细胞内L-GAP的来源仍有待确定。