[骨髓增生异常综合征中CD3(+)CD4(+) T细胞共刺激分子的表达]

[Expressions of costimulatory molecules on CD3(+)CD4(+) T cells in myelodysplastic syndrome].

作者信息

Zeng Hui, Wu De-Pei, Ouyang Jian, He Guang-Sheng, Wnag Xiu-Li

机构信息

Department of Hematology, Nanjing University Medical College, Nanjing 210008, Jiangsu Province, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Oct;16(5):1082-5.

PMID:18928600

Abstract

The study was aimed to detect the expressions of costimulatory molecules on CD3(+)CD4(+) T cells so as to accumulate informations for investigation of mechanism of myelodysplastic syndrome. 11 healthy blood donors as control and 38 patients with MDS de novo were studied. 38 MDS patients were divided into RA/RARS group and RAEB/RAEB-t group according to FAB classification. The expressions of CD28, CD154, CTLA-4, PD-1, CD25 on CD3(+)CD4(+) T cells in peripheral blood were detected by FCM. The results indicated that as compared with normal controls, the expression of CD28 in MDS patients decreased, and CD154 increased. The percentages of CTLA-4, PD-1 and CD25 in MDS were obviously higher than that in normal controls; the differences of CTLA-4, PD-1 and the ratio of CTLA-4/CD28 between RAEB/RAEB-t and RA/RARS were more significant with progressing of MDS. In conclusion, the expressions of costimulatory molecule in MDS patients were abnormal, which may be involved in the pathogenesis of MDS.

摘要

本研究旨在检测CD3(+)CD4(+) T细胞共刺激分子的表达情况，为探讨骨髓增生异常综合征的发病机制积累资料。研究对象包括11名健康献血者作为对照以及38例初发骨髓增生异常综合征患者。38例骨髓增生异常综合征患者根据FAB分型分为RA/RARS组和RAEB/RAEB-t组。采用流式细胞术检测外周血中CD3(+)CD4(+) T细胞上CD28、CD154、CTLA-4、PD-1、CD25的表达。结果显示，与正常对照组相比，骨髓增生异常综合征患者CD28表达降低，CD154表达升高。骨髓增生异常综合征患者CTLA-4、PD-1及CD25的百分比明显高于正常对照组；随着骨髓增生异常综合征病情进展，RAEB/RAEB-t组与RA/RARS组之间CTLA-4、PD-1及CTLA-4/CD28比值的差异更为显著。综上所述，骨髓增生异常综合征患者共刺激分子表达异常，可能参与了骨髓增生异常综合征的发病机制。