骨髓增生异常综合征患者T淋巴细胞早期活化及可溶性肿瘤坏死因子受体的研究

[A study on early activation of T lymphocytes and soluble tumor necrosis factor receptor in patients with myelodysplastic syndrome].

作者信息

Shen Rong, Xu Cong-gao, Li Li-zhen, Zhang Ti, Qin Xue-mei, Li Jie

机构信息

Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, China.

出版信息

Zhonghua Nei Ke Za Zhi. 2003 Dec;42(12):857-60.

PMID:14728878

Abstract

OBJECTIVE

To investigate the expression of T cell early activation marker (CD(69)) on CD(4)(+) and CD(8)(+) lymphocytes in peripheral blood and the levels of soluble tumor necrosis factor receptor 1 (sTNF-R1) and sTNF-R2 in serum and bone marrow in patients with myelodysplastic syndrome (MDS).

METHODS

Whole blood cell culture procedure was applied to activate T lymphocytes with phytohemagglutinin (PHA) (20 mg/L) in vitro. The expression rates of CD(69) on CD(4)(+) and CD(8)(+) lymphocytes at 0 h and 4 h after culture were analyzed with two-color flow cytometry. The levels of sTNF-R1 and sTNF-R2 in serum and bone marrow were measured with ELISA.

RESULTS

There was an increase in the expression rates of CD(69) on CD(4)(+) and CD(8)(+) cells in RA and RAS patients (8.32% and 9.88% respectively) and in the expression rate of CD(69) on CD(8)(+) cells in RAEB and RAEB-T patients (7.92%) before PHA stimulation. CD(69) expression on CD(4)(+) and CD(8)(+) cells were significantly elevated in MDS patients after PHA stimulation (53.46% and 51.63% in RA + RAS; 42.93% and 41.96% in RAEB and RAEB-T) and the expression rate on CD(4)(+) cells was similar to that on CD(8)(+) cells. The levels of the two sTNF-R in MDS patients were elevated. sTNF-R1 in RA and RAS (1.58 +/- 0.68) micro g/L (PB), (2.10 +/- 0.26) micro g/L (BM); sTNF-R2 in RA and RAS (1.41 +/- 0.50) micro g/L (PB), (1.95 +/- 0.64) micro g/L (BM); sTNF-R1 in RAEB and RAEB-T (2.62 +/- 2.55) micro g/L (PB), (3.12 +/- 0.67) micro g/L (BM); sTNF-R2 in RAEB and RAEB-T (1.96 +/- 0.56) micro g/L (PB), (3.09 +/- 0.62) micro g/L (BM). The levels of sTNF-R2 in serum positively correlated to the expression rate of CD(69) on CD(8)(+) cells before PHA stimulation.

CONCLUSION

It is indicate that the increased early activation and activated potentials of T lymphocytes, along with abnormally elevated immunologically active molecules play an important role in immune pathogenesis of patients with MDS.

摘要

目的

探讨骨髓增生异常综合征（MDS）患者外周血中CD4⁺和CD8⁺淋巴细胞上T细胞早期活化标志物（CD69）的表达情况以及血清和骨髓中可溶性肿瘤坏死因子受体1（sTNF-R1）和sTNF-R2的水平。

方法

采用全血细胞培养法，用植物血凝素（PHA）（20mg/L）体外激活T淋巴细胞。用双色流式细胞术分析培养0小时和4小时后CD4⁺和CD8⁺淋巴细胞上CD69的表达率。用ELISA法检测血清和骨髓中sTNF-R1和sTNF-R2的水平。

结果

在PHA刺激前，RA和RAS患者CD4⁺和CD8⁺细胞上CD69的表达率升高（分别为8.32%和9.88%），RAEB和RAEB-T患者CD8⁺细胞上CD69的表达率升高（7.92%）。PHA刺激后，MDS患者CD4⁺和CD8⁺细胞上CD69的表达显著升高（RA+RAS中分别为53.46%和51.63%；RAEB和RAEB-T中分别为42.93%和41.96%），且CD4⁺细胞上的表达率与CD8⁺细胞上的相似。MDS患者两种sTNF-R的水平升高。RA和RAS中sTNF-R1：外周血（1.58±0.68）μg/L，骨髓（2.10±0.26）μg/L；RA和RAS中sTNF-R2：外周血（1.41±0.50）μg/L，骨髓（1.95±0.64）μg/L；RAEB和RAEB-T中sTNF-R1：外周血（2.62±2.55）μg/L，骨髓（3.12±0.67）μg/L；RAEB和RAEB-T中sTNF-R2：外周血（1.96±0.56）μg/L，骨髓（3.09±0.62）μg/L。血清中sTNF-R2水平与PHA刺激前CD8⁺细胞上CD69的表达率呈正相关。