Wang Zhao, Wang Yi-Ni, Feng Cui-Cui, Tian Li-Ping, Chen Xi
Department of Hematology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Oct;16(5):1154-7.
This study was aimed to explore the clinical significance of NK cell activity and serum soluble CD25 (sCD25) level in early diagnosis of the patients with secondary hemophagocytic lymphohistiocytosis (HLH). 38 suspected secondary HLH patients from June 2005 to June 2008 and 25 healthy subjects were enrolled in the study. The NK cell activity in peripheral blood was determined by a released LDH assay, The sCD25 level in serum was detected with ELISA double antibody sandwich assay. The 38 suspected secondary HLH patients were divided into diagnosed and excluded group according to HLH-2004 diagnostic criteria, The NK cell activity and sCD25 level were compared between the two groups. The results showed that 22 out of 38 suspected patients were diagnosed as secondary HLH, the NK cell activity in peripheral blood of these 22 patients was significantly lower than that of healthy control (p < 0.001), the sCD25 level in peripheral blood of these 22 diagnosed patients was higher than that of healthy control (p < 0.05). In conclusion, detection of NK cell activity and sCD25 level may be valuable in the early diagnosis of secondary HLH.
本研究旨在探讨自然杀伤（NK）细胞活性及血清可溶性CD25（sCD25）水平在继发性噬血细胞性淋巴组织细胞增生症（HLH）患者早期诊断中的临床意义。选取2005年6月至2008年6月间38例疑似继发性HLH患者及25例健康对照者纳入研究。采用乳酸脱氢酶（LDH）释放法检测外周血NK细胞活性，应用酶联免疫吸附测定（ELISA）双抗体夹心检测法测定血清sCD25水平。按照HLH - 2004诊断标准将38例疑似继发性HLH患者分为确诊组和排除组，比较两组患者的NK细胞活性及sCD25水平。结果显示，38例疑似患者中22例确诊为继发性HLH，这22例患者外周血NK细胞活性显著低于健康对照（p < 0.001），外周血sCD25水平高于健康对照（p < 0.05）。结论：检测NK细胞活性及sCD25水平对继发性HLH的早期诊断可能具有重要价值。
