Ding Guo-Liang
Dongying Central Blood Station, Dongying 257091, Shandong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Oct;16(5):1196-200.
This study was purposed to investigate the feasibility of cryopreservation platelets under -80 degrees C after stored for 3 days at normal temperature and its clinical use. The platelet count, aggregation functions, adhesive ability, hypotonic shock reaction and CD62p expression of platelets preserved at normal temperature, cryopreserved on same day of collection and after storage for 3 days were detected, and possibility of using platelets cryopreserved after storage for 3 days in clinic was evaluated by comparison of contactable clinical cases. The results indicated that no significant differences in platelet count, hypotonic shock reaction and adhesive ability were found within 3 days of storage (p > 0.05); but differences in aggregation function and CD62p expression were significant (p < 0.05). As compared with platelets cryopreserved on same day of collection, the detected parameters such as platelet count, aggregation function, adhesive ability, hypotonic shock reaction, expression at storage for 3 days did not show significant differences (p > 0.015; CCI value in clinically use of them also did not show significant difference too (p > 0.05). It is concluded that the platelets after storage for 3 days can be cryopreserved, the efficiency of them in clinical use is not significant difference from platelets cryopreserved on same day of collection.
本研究旨在探讨血小板在常温保存3天后于-80℃冷冻保存的可行性及其临床应用。检测常温保存、采集当天冷冻保存及保存3天后冷冻保存的血小板的计数、聚集功能、黏附能力、低渗休克反应及CD62p表达，并通过对比可接触的临床病例评估保存3天后冷冻保存的血小板临床应用的可能性。结果表明，保存3天内血小板计数、低渗休克反应及黏附能力无显著差异（p>0.05）；但聚集功能及CD62p表达差异显著（p<0.05）。与采集当天冷冻保存的血小板相比，保存3天的血小板计数、聚集功能、黏附能力、低渗休克反应、表达等检测指标差异均无统计学意义（p>0.015）；其临床使用中的CCI值差异也无统计学意义（p>0.05）。结论：血小板保存3天后可进行冷冻保存，其临床使用效果与采集当天冷冻保存的血小板无显著差异。
