Boeckh T, Wittmann H G
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, F.R.G.
Biochim Biophys Acta. 1991 Sep 2;1075(1):50-5. doi: 10.1016/0304-4165(91)90073-p.
In order to quantitate and to determine the extent of binding of a heavy atom derivative to ribosomal particles and other components of protein biosynthesis, we prepared a 14C-radioactive labeled monofunctional undecagoldcluster. This cluster can be bound either to exposed sulfhydryl groups at the surface of the ribosomes and its subunits, or to high nucleophilic amino groups of nucleic acids as well as to proteins.
为了定量并确定重原子衍生物与核糖体颗粒及蛋白质生物合成的其他组分的结合程度，我们制备了一种14C放射性标记的单功能十一金簇。该簇可与核糖体及其亚基表面暴露的巯基结合，也可与核酸的高亲核性氨基以及蛋白质结合。
