Hockett Jeffrey L, Dommisch Johanna K, Johnson James D, Cohenca Nestor
Department of Endodontics, School of Dentistry, University of Washington, Seattle, WA; Dental Corps, United States Navy.
Department of Endodontics, School of Dentistry, University of Washington, Seattle, WA; Department of Periodontology, Operative and Preventative Dentistry, University of Bonn, Bonn, Germany.
J Endod. 2008 Nov;34(11):1374-1377. doi: 10.1016/j.joen.2008.07.022. Epub 2008 Sep 3.
The aim of this in vitro study was to determine whether irrigation with apical negative pressure was more effective than traditional positive-pressure irrigation in eradicating Enterococcus faecalis from preshaped root canals. Fifty-four extracted mandibular molars were instrumented to produce either a non-tapered or tapered preparation, sterilized, inoculated with E. faecalis for 30 days, and then randomly assigned into the following groups: group 1--non-tapered preparation and negative-pressure irrigation, group 2--non-tapered preparation and positive-pressure irrigation, group 3--tapered preparation and positive-pressure irrigation, and group 4--tapered preparation and negative-pressure irrigation. Mesial canals were sampled before and after final irrigation and samples incubated aerobically for 48 hours at 37 degrees C. Scanning electron microscopic analysis confirmed dense bacterial colonies in the positive control, consistent with biofilm formation. A statistically significant difference was evident when comparing apical negative-pressure irrigation to positive-pressure irrigation (p=0.004). There was no statistically significant difference in colony-forming units (CFUs) between sizes #35 and #45, nor between tapered and non-tapered preparation. The results of this in vitro study showed that apical negative-pressure irrigation has the potential to achieve better microbial control than traditional irrigation delivery systems.
本体外研究的目的是确定根尖负压冲洗在根除预成根管内粪肠球菌方面是否比传统的正压冲洗更有效。54颗拔除的下颌磨牙经预备形成非锥形或锥形根管，消毒后接种粪肠球菌30天，然后随机分为以下几组：第1组——非锥形根管预备和负压冲洗，第2组——非锥形根管预备和正压冲洗，第3组——锥形根管预备和正压冲洗，第4组——锥形根管预备和负压冲洗。在最终冲洗前后对近中根管取样，并将样本在37℃有氧条件下培养48小时。扫描电子显微镜分析证实阳性对照中有密集的细菌菌落，与生物膜形成一致。将根尖负压冲洗与正压冲洗进行比较时，差异具有统计学意义（p = 0.004）。在35号和45号器械之间以及锥形和非锥形根管预备之间，菌落形成单位（CFU）没有统计学上的显著差异。本体外研究结果表明，根尖负压冲洗比传统冲洗系统更有潜力实现更好的微生物控制。
