使用带有内标和高Q值双调谐线圈的核磁共振波谱法对31P肌肉代谢物进行绝对定量分析。

Absolute quantification of 31P muscle metabolites using NMRS with an internal standard and a high-Q, double-tuned coil.

作者信息

De Bisschop E, Annaert G, Luypaert R, Coremans J, Osteaux M

机构信息

Biomedical MR Unit, PRIMIS, Brussels, Belgium.

出版信息

Biochim Biophys Acta. 1991 Sep 3;1094(2):147-52. doi: 10.1016/0167-4889(91)90002-f.

DOI:10.1016/0167-4889(91)90002-f

PMID:1892897

Abstract

31P-nuclear magnetic resonance spectroscopy (NMRS) is used to determine absolute concentrations of 31P metabolites in rat muscle. A technique exploiting tissue water 1H as internal concentration reference and a highly sensitive, double-tuned coil is described and evaluated experimentally. On KH2PO4 solutions of known concentration and varying coil loading it is shown to allow neutralization of systematic errors due to conductive losses, which normally range up to 20% or more. In vivo application in the determination of the absolute concentrations of ATP, PCr and Pi in eight rat thighs yields results that are in good agreement with literature values.

摘要

31P核磁共振波谱法（NMRS）用于测定大鼠肌肉中31P代谢物的绝对浓度。本文描述并通过实验评估了一种利用组织水1H作为内部浓度参考的技术以及一个高灵敏度的双调谐线圈。在已知浓度且线圈负载不同的KH2PO4溶液上进行实验，结果表明该技术能够抵消通常高达20%或更高的由于传导损耗导致的系统误差。在对八只大鼠大腿中ATP、磷酸肌酸（PCr）和无机磷（Pi）绝对浓度的体内测定中，所得结果与文献值高度吻合。