Bonn Stefan, Furlong Eileen E M
European Molecular Biology Laboratory, Heidelberg, Germany.
Curr Opin Genet Dev. 2008 Dec;18(6):513-20. doi: 10.1016/j.gde.2008.09.005. Epub 2008 Oct 16.
Understanding how regulatory networks initiate, maintain and synchronise transcriptional states remains a fundamental goal of developmental biology. Complex patterns of spatio-temporal gene expression are generated through the combined inputs of signalling and transcriptional networks converging on cis-regulatory modules (CRMs). Detailed studies in Drosophila, using transgenic reporter assays and mutagenesis analysis, have dissected the regulatory logic of a number of CRMs. These data have recently been complemented by genome-wide maps of transcription factor binding, revealing an unprecedented view of CRM occupancy and network complexity. The synthesis of data for three well-characterised Drosophila developmental networks reveals emerging themes at both a CRM and a cis-regulatory network level.
理解调控网络如何启动、维持和同步转录状态仍然是发育生物学的一个基本目标。时空基因表达的复杂模式是通过信号传导网络和转录网络汇聚到顺式调控模块（CRM）上的联合输入而产生的。在果蝇中，利用转基因报告基因检测和诱变分析进行的详细研究，剖析了许多CRM的调控逻辑。这些数据最近得到了全基因组转录因子结合图谱的补充，揭示了CRM占据情况和网络复杂性的前所未有的景象。对三个特征明确的果蝇发育网络的数据综合揭示了在CRM和顺式调控网络层面出现的新主题。
