Yuan Y, Cong C, Zhang J, Wei L, Li S, Chen Y, Tan W, Cheng J, Li Y, Zhao X, Lu Y
Key Lab of Transplant Engineering and Immunology, Ministry of Health, P R China.
Transplant Proc. 2008 Oct;40(8):2571-4. doi: 10.1016/j.transproceed.2008.08.017.
Hypoxia and reoxygenation (H/R)-induced damage often happens soon after islets are transplantation. The process of islet isolation and purification causes the rapid onset of hypoxia. We sought to develop a functional scaffold to sustain the structure and function of islets as well as to recover some of the surface molecules damaged during isolation, seeking to improve islet transplantation outcomes. Self-assembling peptide nanofiber (SAPNF), a new type of substrate has been shown to be an excellent biological material for neuronal cell culture and tissue engineering in animals. In this study, we investigated the protective effect of SAPNF on damage to rat islets. Freshly prepared rat islets from male Sprague-Dawley rats were seeded in plates coated with (SAPNF-treated group) or without (control group) SAPNF. The islets were then divided into two groups culture under normoxia for 7 days versus exposure to hypoxia (< 1% O2) for 6 hours followed by reoxygenation for 24 hours. The results showed that SAPNF exhibited improving effects on viability and function of cultured islets, protecting the one from H/R-induced damage. In both groups, the stimulation index of SAPNF-treated groups were about two times the controls. SAPNF treatment decreased apoptotic rates of islet cells. These results suggested the usefulness of SAPNF to maintain the viability and function of rat pancreatic islets. SAPNF may be a potential scaffold for clinical islet transplantation.
缺氧复氧（H/R）诱导的损伤常在胰岛移植后不久发生。胰岛分离纯化过程会导致缺氧迅速发生。我们试图开发一种功能性支架，以维持胰岛的结构和功能，并恢复分离过程中受损的一些表面分子，旨在改善胰岛移植结果。自组装肽纳米纤维（SAPNF）作为一种新型底物，已被证明是动物神经元细胞培养和组织工程的优良生物材料。在本研究中，我们研究了SAPNF对大鼠胰岛损伤的保护作用。将雄性Sprague-Dawley大鼠新鲜制备的胰岛接种在涂有（SAPNF处理组）或未涂有（对照组）SAPNF的培养皿中。然后将胰岛分为两组，一组在常氧下培养7天，另一组暴露于缺氧（<1%O2）6小时，随后复氧24小时。结果表明，SAPNF对培养的胰岛的活力和功能具有改善作用，保护其免受H/R诱导的损伤。在两组中，SAPNF处理组的刺激指数约为对照组的两倍。SAPNF处理降低了胰岛细胞的凋亡率。这些结果表明SAPNF有助于维持大鼠胰岛的活力和功能。SAPNF可能是临床胰岛移植的潜在支架。
