Wang Tao, He Guangzhi, Yang Guangyou, Fei Yuxiang, Zhang Zhihe, Wang Chengdong, Yang Zhi, Lan Jingchao, Luo Li, Liu Li
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Vaccine. 2008 Dec 9;26(52):6919-24. doi: 10.1016/j.vaccine.2008.09.079. Epub 2008 Oct 16.
The gene Bs-Ag3 enconding a antigen of 37kDa from Baylisascaris schroederi (giant panda isolates), as well as the recombinant Bs-Ag3, obtained by cloning and expression of the Bs-Ag3 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in BALB/c mice against L3-challenge infection in a mouse-B. schroederi model. There was a significant reduction (62.91%) of mice vaccinated with rBs-Ag3 coupled with Freund's complete adjuvant (FCA) in recovery of challenged B. schroederi L3 compared with either controls or mice vaccinated with FCA alone. Our data indicate recombinant Bs-Ag3 may be a potential target as a vaccine antigen for giant panda ascariasis.
编码来自斯氏狸殖吸虫（大熊猫分离株）37kDa抗原的基因Bs-Ag3，以及通过在异源宿主大肠杆菌BL-21(DE3)中克隆和表达Bs-Ag3基因获得的重组Bs-Ag3，用于评估它们在小鼠-斯氏狸殖吸虫模型中诱导BALB/c小鼠针对L3攻击感染产生免疫保护反应的能力。与对照组或仅接种弗氏完全佐剂（FCA）的小鼠相比，接种rBs-Ag3与弗氏完全佐剂（FCA）联合疫苗的小鼠在感染斯氏狸殖吸虫L3后的恢复情况有显著降低（62.91%）。我们的数据表明，重组Bs-Ag3可能是大熊猫蛔虫病疫苗抗原的潜在靶点。
