人增殖细胞核抗原（PCNA）与含人DNA聚合酶ι非经典增殖细胞核抗原相互作用基序（PIP-box）序列的肽形成复合物的初步晶体学研究
Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase iota.
作者信息
Hishiki Asami, Shimizu Toshiyuki, Hanafusa Tomo, Ohmori Haruo, Sato Mamoru, Hashimoto Hiroshi
机构信息
International Graduate School of Arts and Sciences, Yokohama City University, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt 10):954-6. doi: 10.1107/S1744309108028522. Epub 2008 Sep 30.
Human DNA polymerase iota (Poliota) is one of the Y-family DNA polymerases involved in translesion synthesis (TLS), which allows continued replication at damaged DNA templates. Poliota has a noncanonical PCNA-interacting protein box (PIP-box) within an internal region of the protein. Poliota activity is stimulated by PCNA binding through the noncanonical PIP-box. To clarify the interaction of PCNA with the noncanonical PIP-box of Poliota, PCNA and a Poliota peptide carrying the noncanonical PIP-box complex have been cocrystallized. The crystal belongs to space group C2, with unit-cell parameters a = 167.1, b = 68.7, c = 90.0 A, beta = 95.1 degrees . Structural analysis by molecular replacement is in progress.
人类DNA聚合酶ι（Poliota）是参与跨损伤合成（TLS）的Y家族DNA聚合酶之一，TLS可使受损DNA模板继续进行复制。Poliota在该蛋白质的内部区域有一个非典型的增殖细胞核抗原（PCNA）相互作用蛋白框（PIP框）。通过非典型PIP框与PCNA的结合可刺激Poliota的活性。为阐明PCNA与Poliota非典型PIP框之间的相互作用，已使PCNA与携带非典型PIP框复合物的Poliota肽共结晶。该晶体属于空间群C2，晶胞参数为a = 167.1、b = 68.7、c = 90.0 Å，β = 95.1°。正在通过分子置换进行结构分析。
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