Soda Kosuke, Ozaki Hiroichi, Sakoda Yoshihiro, Isoda Norikazu, Haraguchi Yoshinari, Sakabe Saori, Kuboki Noritaka, Kishida Noriko, Takada Ayato, Kida Hiroshi
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan.
Arch Virol. 2008;153(11):2041-8. doi: 10.1007/s00705-008-0226-3. Epub 2008 Oct 21.
In order to prepare H5N1 influenza virus vaccine, the hemagglutinins (HAs) of 14 H5 virus isolates from water birds in Asia were antigenically and genetically analyzed. Phylogenetic analysis of the H5 HA genes revealed that 13 isolates belong to Eurasian and the other one to North American lineages. Each of the deduced amino acid sequences of the HAs indicated a non-pathogenic profile. Antigenic analysis using a panel of monoclonal antibodies recognizing six different epitopes on the HA of A/duck/Pennsylvania/10218/1984 (H5N2) and chicken antiserum to an H5N1 reassortant strain generated between A/duck/Mongolia/54/2001 (H5N2) and A/duck/Mongolia/47/2001 (H7N1), [R(Dk/Mong-Dk/Mong) (H5N1)] showed that the HAs of highly pathogenic avian influenza (HPAI) viruses currently circulating in Asia were antigenically closely related to those of the present isolates from water birds. Mice subcutaneously injected with formalin-inactivated R(Dk/Mong-Dk/Mong) were protected from challenge with 100 mouse lethal dose of A/Viet Nam/1194/2004 (H5N1). The present results support the notion that the H5 isolates and the reassortant H5N1 strain should be useful for vaccine preparation.
为制备H5N1流感病毒疫苗，对从亚洲水鸟中分离出的14株H5病毒的血凝素（HA）进行了抗原性和基因分析。对H5 HA基因的系统发育分析表明，13株分离株属于欧亚谱系，另一株属于北美谱系。每个HA推导的氨基酸序列均显示出非致病特征。使用一组识别A/鸭/宾夕法尼亚/10218/1984（H5N2）HA上六个不同表位的单克隆抗体以及针对A/鸭/蒙古/54/2001（H5N2）和A/鸭/蒙古/47/2001（H7N1）之间产生的H5N1重配株[R（鸭/蒙古-鸭/蒙古）（H5N1）]的鸡抗血清进行抗原分析，结果表明，目前在亚洲流行的高致病性禽流感（HPAI）病毒的HA与当前从水鸟中分离出的毒株在抗原性上密切相关。皮下注射福尔马林灭活的R（鸭/蒙古-鸭/蒙古）的小鼠在受到100个小鼠致死剂量的A/越南/1194/2004（H5N1）攻击时得到了保护。目前的结果支持这样一种观点，即H5分离株和重配H5N1毒株应可用于疫苗制备。
