Cahill P, Foster K, Mahan D E
Gene-Trak Systems, Framingham, MA 01701.
Clin Chem. 1991 Sep;37(9):1482-5.
The polymerase chain reaction (PCR) and Q beta replicase are two methods in which nucleic acid polymerases are used for amplification. Although these approaches share many similar problems concerning target contamination and probe specificity, they differ dramatically in their mechanisms of action and modes of application. The PCR method amplifies target sequences between two priming oligonucleotides and in essence amplifies a portion of the analyte. Q beta replicase, on the other hand, amplifies a specific template molecule hybridized to target sequences and therefore amplifies a signal component of the system. For this reason, Q beta replicase amplification has applications in areas other than for the detection of nucleic acid sequences. The requirements for application and the advantages of both PCR and Q beta replicase amplification are reviewed.
聚合酶链反应（PCR）和Qβ复制酶是两种使用核酸聚合酶进行扩增的方法。尽管这些方法在靶标污染和探针特异性方面存在许多相似问题，但它们在作用机制和应用方式上有显著差异。PCR方法在两个引物寡核苷酸之间扩增靶序列，本质上是扩增分析物的一部分。另一方面，Qβ复制酶扩增与靶序列杂交的特定模板分子，因此扩增系统的信号成分。出于这个原因，Qβ复制酶扩增在核酸序列检测以外的领域也有应用。本文综述了PCR和Qβ复制酶扩增的应用要求及优势。
