通过肌动蛋白踏车运动和分子马达进行的蛋白质定位调节静纤毛的形状和踏车运动速率。
Protein localization by actin treadmilling and molecular motors regulates stereocilia shape and treadmilling rate.
作者信息
Naoz Moshe, Manor Uri, Sakaguchi Hirofumi, Kachar Bechara, Gov Nir S
机构信息
Department of Chemical Physics, Weizmann Institute of Science, Rehovot, Israel.
出版信息
Biophys J. 2008 Dec 15;95(12):5706-18. doi: 10.1529/biophysj.108.143453. Epub 2008 Oct 20.
Abstract
We present a physical model that describes the active localization of actin-regulating proteins inside stereocilia during steady-state conditions. The mechanism of localization is through the interplay of free diffusion and directed motion, which is driven by coupling to the treadmilling actin filaments and to myosin motors that move along the actin filaments. The resulting localization of both the molecular motors and their cargo is calculated, and is found to have an exponential (or steeper) profile. This localization can be at the base (driven by actin retrograde flow and minus-end myosin motors), or at the stereocilia tip (driven by plus-end myosin motors). The localization of proteins that influence the actin depolymerization and polymerization rates allow us to describe the narrow shape of the stereocilia base, and the observed increase of the actin polymerization rate with the stereocilia height.
摘要
我们提出了一个物理模型，该模型描述了在稳态条件下肌动蛋白调节蛋白在静纤毛内的主动定位。定位机制是通过自由扩散和定向运动的相互作用，这种相互作用由与肌动蛋白踏车丝以及沿肌动蛋白丝移动的肌球蛋白马达的耦合驱动。计算了分子马达及其货物的最终定位，发现其具有指数（或更陡峭）分布。这种定位可以在基部（由肌动蛋白逆行流和负端肌球蛋白马达驱动），也可以在静纤毛尖端（由正端肌球蛋白马达驱动）。影响肌动蛋白解聚和聚合速率的蛋白质的定位使我们能够描述静纤毛基部的狭窄形状，以及观察到的肌动蛋白聚合速率随静纤毛高度的增加。
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本文引用的文献
1
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Semin Cell Dev Biol. 2008 Dec;19(6):502-10. doi: 10.1016/j.semcdb.2008.07.006. Epub 2008 Jul 25.
2
Dynamic compartmentalization of protein tyrosine phosphatase receptor Q at the proximal end of stereocilia: implication of myosin VI-based transport.蛋白酪氨酸磷酸酶受体Q在静纤毛近端的动态区室化：基于肌球蛋白VI的转运的意义
Cell Motil Cytoskeleton. 2008 Jul;65(7):528-38. doi: 10.1002/cm.20275.
3
Ena/VASP proteins capture actin filament barbed ends.埃娜/血管舒缩刺激磷蛋白（Ena/VASP）家族蛋白可捕捉肌动蛋白丝的带刺末端。
J Biol Chem. 2008 Apr 11;283(15):9814-9. doi: 10.1074/jbc.M710475200. Epub 2008 Feb 18.
4
Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.Arp2/3复合体对神经元细胞中丝状伪足的形成、生长锥运动和神经突发生很重要。
Mol Biol Cell. 2008 Apr;19(4):1561-74. doi: 10.1091/mbc.e07-09-0964. Epub 2008 Feb 6.
5
The kinase domain alters the kinetic properties of the myosin IIIA motor.激酶结构域改变了肌球蛋白IIIA马达的动力学特性。
Biochemistry. 2008 Feb 26;47(8):2485-96. doi: 10.1021/bi7021574. Epub 2008 Jan 30.
6
The many faces of actin: matching assembly factors with cellular structures.肌动蛋白的多样面貌：将组装因子与细胞结构相匹配。
Nat Cell Biol. 2007 Oct;9(10):1110-21. doi: 10.1038/ncb1007-1110.
7
Intrinsic dynamic behavior of fascin in filopodia.丝状伪足中肌动蛋白丝束蛋白的内在动态行为。
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10
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