Wang Lijuan, Li Xiaofeng, Chen Shuangyan, Liu Gongshe
Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, People's Republic of China.
Biotechnol Lett. 2009 Feb;31(2):313-9. doi: 10.1007/s10529-008-9864-5. Epub 2008 Oct 21.
Leymus chinensis is an important grassland perennial grass. However, its drought tolerance requires to be improved. LEA (late embryogenesis abundant) genes are believed to confer resistance to drought and water deficiency. Using Agrobacterium-mediated transformation, a wheat LEA gene, TaLEA(3), was integrated into L. chinensis. The transgenic lines showed enhanced growth ability under drought stress during which transgenic lines had increased the relative water content, leaf water potential, relative average growth rate, but decreased the malondialdehyde content compared with the non-transgenic plant. Thus, transgenic breeding is an efficient approach to enhance drought tolerance in L. chinensis.
羊草是一种重要的草原多年生草本植物。然而,其耐旱性有待提高。胚胎后期丰富(LEA)基因被认为赋予对干旱和水分亏缺的抗性。利用农杆菌介导的转化方法,将一个小麦LEA基因TaLEA(3)导入羊草中。转基因株系在干旱胁迫下表现出增强的生长能力,在此期间,与非转基因植株相比,转基因株系的相对含水量、叶片水势、相对平均生长速率增加,而丙二醛含量降低。因此,转基因育种是提高羊草耐旱性的一种有效方法。