Hinshaw L B, Archer L T, Beller B K, White G L, Schroeder T M, Holmes D D
Am J Physiol. 1977 Aug;233(2):E71-9. doi: 10.1152/ajpendo.1977.233.2.E71.
The present study was conducted to explore influences modifying glucose uptake in canine blood administered LD100 E. coli endotoxin. Particular emphasis was given to assay the role of the white blood cell (WBC) in glucose utilization. Significant increases in glucose uptake and lactic acid production, attributed to increased activity of the WBC, were observed 1-3 h after endotoxin was added to blood in vitro. Although a net increase in glucose utilization was noted, endotoxin simultaneously exerted adverse effects by depressing glucose uptake below predicted values (Q10 = 2.12 with LD100 endotoxin vs. 2.78 in saline controls) and increasing WBC mortality rate. Blood from dogs pretreated with sublethal doses of endotoxin in vivo utilized glucose at an accelerated rate when subjected to endotoxin in vitro. Excess glucose was consumed because of elevated numbers of white blood cells although additional glucose requirements after endotoxin were independent of temperature between the ranges of 34-41 degrees C. All animals pretreated with daily sublethal injections of endotoxin for 3 days survived superlethal doses of endotoxin.
本研究旨在探讨影响犬血液中给予致死剂量100%大肠杆菌内毒素后葡萄糖摄取的因素。特别着重于测定白细胞（WBC）在葡萄糖利用中的作用。体外向内毒素加入血液后1 - 3小时，观察到由于白细胞活性增加导致葡萄糖摄取和乳酸生成显著增加。尽管注意到葡萄糖利用有净增加，但内毒素同时通过将葡萄糖摄取压低至预测值以下（致死剂量100%内毒素时Q10 = 2.12，而生理盐水对照组为2.78）以及增加白细胞死亡率产生不利影响。体内经亚致死剂量内毒素预处理的犬的血液，在体外接受内毒素时以加速速率利用葡萄糖。由于白细胞数量增加，过量的葡萄糖被消耗，尽管内毒素后额外的葡萄糖需求在34 - 41摄氏度范围内与温度无关。所有每日接受亚致死剂量内毒素注射3天的预处理动物均存活于超致死剂量的内毒素。
