Kou Zheng, Zhang Zhong, Chen Shengliang, Fan Zhaojun, Tang Shuang, Zhao Lin, Li Tianxian
Department of System Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Avian Dis. 2008 Sep;52(3):451-4. doi: 10.1637/8223-012408-Reg.1.
Budgerigar fledgling disease is an acute viral infectious disease caused by avian polyomavirus (APV). In this study, 34 liver tissue samples of young, dead budgerigar with typical symptoms were collected in 2004. All the samples had positive polymerase chain reaction (PCR) test based on the VP1 specific primers. VP1 genes of these samples were sequenced and had high similarities to each other (99%-100%). A strain (HBYM02) was isolated and sequenced. As shown in the phylogenetic tree, there are two branches. One branch was composed by strains isolated from Passeriformes, and the other was composed only by one strain isolated from Falconiformes. The genome similarities between our isolate and other reported isolates were very high (> 99%), and the evolution distances in the phylogenetic tree were very short (< 0.005), which suggests that APV in China has the same genotype as those in other regions. The results will be useful for the diagnoses of, and vaccine development for, APV.
虎皮鹦鹉雏鸟病是一种由禽多瘤病毒（APV）引起的急性病毒性传染病。在本研究中，2004年收集了34只具有典型症状的死亡幼龄虎皮鹦鹉的肝脏组织样本。所有样本基于VP1特异性引物的聚合酶链反应（PCR）检测均呈阳性。对这些样本的VP1基因进行了测序，它们彼此之间具有高度相似性（99%-100%）。分离出一个毒株（HBYM02）并进行了测序。如系统发育树所示，有两个分支。一个分支由从雀形目分离出的毒株组成，另一个分支仅由从隼形目分离出的一个毒株组成。我们分离的毒株与其他已报道的分离株之间的基因组相似性非常高（>99%），并且在系统发育树中的进化距离非常短（<0.005），这表明中国的APV与其他地区的APV具有相同的基因型。这些结果将有助于APV的诊断和疫苗研发。
