Occhipinti Paola, Griffiths Peter C
School of Chemistry, Cardiff University, Main Building, Park Place, Cardiff CF10 3AT, UK.
Adv Drug Deliv Rev. 2008 Dec 14;60(15):1570-82. doi: 10.1016/j.addr.2008.08.006. Epub 2008 Sep 25.
Mucus, a thick and slimy secretion produced by submucosal cells, covers many epithelial surfaces in mammalian organs and prevents foreign particles that enter the body from accessing cells. However, the mucus layer also represents a potential barrier to the efficient delivery of nano-sized drug delivery systems (polyplexes, lipoplexes, particles) to the underlying mucosal epithelium. Many studies have considered the ability of nano-sized particles and polymers to diffuse within the mucosal network using a range of different techniques, including multiple-particle tracking (MPT), diffusion chamber studies and fluorescence recovery after photobleaching (FRAP). This review highlights the current understanding of the interaction of the diffusion of nano-sized structures within mucosal networks. Moreover, this article presents an introduction to pulsed-gradient spin-echo NMR (PGSE-NMR), a potential new tool to investigate the mobility of molecular species through mucosal networks and related biological gels.
黏液是由黏膜下细胞产生的一种浓稠且黏滑的分泌物，覆盖着哺乳动物器官中的许多上皮表面，可防止进入体内的外来颗粒接触细胞。然而，黏液层对于将纳米级药物递送系统（多聚体、脂质体、颗粒）有效递送至下层黏膜上皮来说，也是一个潜在的障碍。许多研究使用了一系列不同技术，包括多粒子追踪（MPT）、扩散室研究和光漂白后荧光恢复（FRAP），来考察纳米级颗粒和聚合物在黏膜网络内扩散的能力。本综述着重介绍了目前对纳米级结构在黏膜网络内扩散相互作用的理解。此外，本文还介绍了脉冲梯度自旋回波核磁共振（PGSE-NMR），这是一种用于研究分子通过黏膜网络及相关生物凝胶迁移率的潜在新工具。
