Deschout Hendrik, Raemdonck Koen, Demeester Jo, De Smedt Stefaan C, Braeckmans Kevin
Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium.
Pharm Res. 2014 Feb;31(2):255-70. doi: 10.1007/s11095-013-1146-9. Epub 2013 Sep 10.
Fluorescence recovery after photobleaching (FRAP) is a fluorescence microscopy technique that has attracted a lot of interest in pharmaceutical research during the last decades. The main purpose of FRAP is to measure diffusion on a micrometer scale in a non-invasive and highly specific way, making it capable of measurements in complicated biomaterials, even in vivo. This has proven to be very useful in the investigation of drug diffusion inside different tissues of the body and in materials for controlled drug delivery. FRAP has even found applications for the improvement of several medical therapies and in the field of diagnostics. In this review, an overview is given of the different applications of FRAP in pharmaceutical research, together with essential guidelines on how to perform and analyse FRAP experiments.
光漂白后荧光恢复（FRAP）是一种荧光显微镜技术，在过去几十年中引起了药物研究领域的广泛关注。FRAP的主要目的是以非侵入性和高度特异性的方式测量微米尺度上的扩散，使其能够在复杂的生物材料中进行测量，甚至在体内也可以。事实证明，这在研究药物在人体不同组织内的扩散以及用于控制药物递送的材料方面非常有用。FRAP甚至在改善多种医学治疗方法以及诊断领域中得到了应用。在本综述中，概述了FRAP在药物研究中的不同应用，以及进行和分析FRAP实验的基本指南。
