Jia Bao-Zhu, Liu Yan-Yan, Chen Feng-Yan, Tongchai Phannika, Yadoung Sumed, Wongta Anurak, Xu Zhen-Lin, Luo Lin, Hongsibsong Surat
School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China.
Food Chem X. 2025 Jun 3;28:102613. doi: 10.1016/j.fochx.2025.102613. eCollection 2025 May.
Dichlorvos (DDVP), a widely used yet environmentally hazardous organophosphate pesticide, necessitates rapid field monitoring due to increasing regulatory restrictions. In this study, we firstly present a gold-nanoparticle-based lateral flow immunochromatographic assay (LFIA) employing a highly specific anti-DDVP monoclonal antibody for onsite DDVP detection within 20 min. The optimized LFIA exhibited exceptional analytical performance, with limits of detection spanning 16 μg/kg to 108 μg/kg across six fruit and vegetable matrices-all below the established maximum residue limits in foods. Method validation with spiked fruit and vegetable samples showed recovery rates of 87.3-109.4 % and 89.2-103.3 %, respectively, with coefficients of variation below 8.3 % and 7.9 %, respectively, confirming the accuracy and precision of the assay. Notably, LFIA exhibited good agreement with standard GC-MS/MS analysis when applied to 144 authentic samples, further validating its reliability for real-world applications. This LFIA offers key advantages including portability, cost-effectiveness, and compatibility with regulatory thresholds, making it an ideal alternative to conventional instrumental methods for routine pesticide residue screening in food safety supervision settings.
敌敌畏（DDVP）是一种广泛使用但对环境有害的有机磷农药，由于监管限制日益严格，需要进行快速现场监测。在本研究中，我们首先提出了一种基于金纳米颗粒的侧向流动免疫色谱分析方法（LFIA），该方法使用高度特异性的抗DDVP单克隆抗体在20分钟内进行现场DDVP检测。优化后的LFIA表现出卓越的分析性能，在六种水果和蔬菜基质中的检测限为16μg/kg至108μg/kg，均低于食品中既定的最大残留限量。对加标水果和蔬菜样品的方法验证表明，回收率分别为87.3-109.4%和89.2-103.3%，变异系数分别低于8.3%和7.9%，证实了该分析方法的准确性和精密度。值得注意的是，当应用于144个真实样品时，LFIA与标准GC-MS/MS分析结果具有良好的一致性，进一步验证了其在实际应用中的可靠性。这种LFIA具有便携性、成本效益高以及与监管阈值兼容等关键优势，使其成为食品安全监管环境中常规农药残留筛查的传统仪器方法的理想替代方案。
