Phytopathology. 2007 Apr;97(4):523-31. doi: 10.1094/PHYTO-97-4-0523.
ABSTRACT The endophyte Piriformospora indica colonizes roots of a range of host plants and increases biomass production and resistance to fungal pathogens and, thus has been considered a biocontrol fungus. However, the field performance of this fungus has not yet been tested in temperate climates. Therefore, we evaluated the performance of this fungus in different substrata under greenhouse and practical field conditions. Roots of winter wheat were colonized efficiently, and biomass was particularly increased on poor substrata. In greenhouse experiments, symptom severity of a typical leaf (Blumeria graminis f. sp. tritici), stem base (Pseudocercosporella herpotrichoides), and root (Fusarium culmorum) pathogen was reduced significantly. However, in field experiments, symptoms caused by the leaf pathogen did not differ in Piriformospora indica-colonized compared with control plants. In the field, Pseudocercosporella herpotrichoides disease severity was significantly reduced in plants colonized by the endophyte. Increased numbers of sheath layers and hydrogen peroxide concentrations after B. graminis attack were detected in Piriformospora indica-colonized plants, suggesting that root colonization causes induction of systemic resistance or priming of the host plant. Although the endophyte is not well suited for growth at Central European temperature conditions, it remains to be shown whether P. indica is more suitable for tropical or subtropical farming.
摘要 内寄生真菌梨形侧耳能够定殖于一系列宿主植物的根部，增加生物量的产生并提高宿主植物对真菌病原体的抗性，因此被认为是一种生防真菌。然而，这种真菌在温带气候下的田间性能尚未得到测试。因此，我们评估了该真菌在温室和实际田间条件下不同基质中的表现。冬季小麦的根部能够被有效地定殖，在贫瘠的基质上生物量的增加尤其明显。在温室实验中，典型叶片（禾谷布氏黑粉菌）、茎基部（拟禾谷多腔菌）和根部（禾谷镰刀菌）病原体的症状严重度显著降低。然而，在田间实验中，与对照植物相比，梨形侧耳定殖的植物叶片病原体引起的症状没有差异。在田间，内寄生真菌的定殖显著降低了拟禾谷多腔菌病害的严重度。在梨形侧耳定殖的植物中，检测到禾谷布氏黑粉菌攻击后鞘层数和过氧化氢浓度增加，表明根部定殖导致了系统抗性的诱导或宿主植物的启动。尽管该内生真菌在中欧温度条件下的生长不太适应，但仍需证明梨形侧耳是否更适合热带或亚热带农业。
