Roylawar Praveen, Khandagale Kiran, Nanda Satyabrata, Soumia Parakkattu Sulochanan, Jadhav Sangita, Mahajan Vijay, Gawande Suresh
ICAR-Directorate of Onion and Garlic Research, Pune, India.
Department of Botany, S.N. Arts, D.J.M. Commerce and B.N.S. Science College, Sangamner, Maharashtra, India.
Front Microbiol. 2023 Jul 26;14:1190942. doi: 10.3389/fmicb.2023.1190942. eCollection 2023.
Plant-endophyte symbiosis influences plant defense and growth. is a root endophyte that promotes growth and induces tolerance against biotic and abiotic stress in plants. In this study, we examined the effect of colonization on herbivore () resistance of onion ( L.). We found that colonization of in the roots of onion significantly reduced the feeding damage of leaves by larvae, and also resulted in a reduction in weight gain of the larvae when fed on plants. This enhanced resistance is a result of modulation of antioxidant and defense enzymes/genes in the host by mutualism. Specifically, the activities of enzymes such as Superoxide dismutase, peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, and HO content were significantly higher in the early stages of feeding in the colonized plants compared to the non-colonized counterparts. Similarly, defense genes also showed modulation in response to this tripartite interaction of onion - mutualism and herbivory. The hierarchical cluster analysis and principal component analysis indicated a clear difference in the onion biochemical responses, which is due to the symbiosis. Our investigation demonstrates that onion- symbiosis significantly decreases chewing injury by efficiently modulating antioxidant and defense enzyme activities and gene expression in response to herbivory. Therefore, can be used as a potential biocontrol agent for sustainable management of this important pest of Alliums.
植物-内生菌共生影响植物防御和生长。[内生菌名称]是一种促进植物生长并诱导植物对生物和非生物胁迫产生耐受性的根内生菌。在本研究中，我们研究了[内生菌名称]定殖对洋葱（葱属植物）抗食草动物（[食草动物名称]）能力的影响。我们发现，[内生菌名称]在洋葱根部定殖显著降低了[食草动物名称]幼虫对叶片的取食损伤，并且在以定殖了[内生菌名称]的植物为食时，幼虫的体重增加也有所减少。这种增强的抗性是[内生菌名称]与宿主互利共生调节宿主抗氧化和防御酶/基因的结果。具体而言，与未定殖的对照相比，在定殖了[内生菌名称]的植物中，超氧化物歧化酶、过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶等酶的活性以及过氧化氢含量在[食草动物名称]取食的早期阶段显著更高。同样，防御基因也因洋葱-[内生菌名称]互利共生和[食草动物名称]取食的这种三方相互作用而表现出调节。层次聚类分析和主成分分析表明，由于[内生菌名称]共生，洋葱的生化反应存在明显差异。我们的研究表明，洋葱-[内生菌名称]共生通过有效调节抗氧化和防御酶活性以及响应[食草动物名称]取食的基因表达，显著降低了咀嚼损伤。因此，[内生菌名称]可作为一种潜在的生物防治剂，用于对葱属植物这种重要害虫的可持续管理。
