Phytopathology. 2006 Oct;96(10):1081-91. doi: 10.1094/PHYTO-96-1081.
ABSTRACT Pathogenicity of 75 strains of Xanthomonas albilineans from Guadeloupe was assessed by inoculation of sugarcane cv. B69566, which is susceptible to leaf scald, and 19 of the strains were selected as representative of the variation in pathogenicity observed based on stalk colonization. In vitro production of albicidin varied among these 19 strains, but the restriction fragment length polymorphism pattern of their albicidin biosynthesis genes was identical. Similarly, no genomic variation was found among strains by pulsed-field gel electrophoresis. Some variation among strains was found by amplified fragment length polymorphism, but no relationship between this genetic variation and variation in pathogenicity was found. Only 3 (pilB, rpfA, and xpsE) of 40 genes involved in pathogenicity of bacterial species closely related to X. albilineans could be amplified by polymerase chain reaction from total genomic DNA of all nine strains tested of X. albilineans differing in pathogenicity in Guadeloupe. Nucleotide sequences of these genes were 100% identical among strains, and a phylogenetic study with these genes and housekeeping genes efp and ihfA suggested that X. albilineans is on an evolutionary road between the X. campestris group and Xylella fastidiosa, another vascular plant pathogen. Sequencing of the complete genome of Xanthomonas albilineans could be the next step in deciphering molecular mechanisms involved in pathogenicity of X. albilineans.
摘要 从瓜德罗普岛分离的 75 株黄单胞菌致病性通过接种易感叶枯病的甘蔗品种 B69566 进行评估，根据茎干定殖观察到的致病性变化选择其中 19 株作为代表性菌株。19 株菌株中 albicidin 的体外产量存在差异，但 albicidin 生物合成基因的限制性片段长度多态性模式相同。同样，通过脉冲场凝胶电泳未发现菌株间的基因组变异。菌株间通过扩增片段长度多态性发现了一些变异，但未发现这种遗传变异与致病性变异之间存在关系。在所测试的 9 株致病性不同的瓜德罗普岛黄单胞菌总基因组 DNA 中，仅能通过聚合酶链反应扩增与致病性密切相关的 40 个细菌种属相关基因中的 3 个（pilB、rpfA 和 xpsE）。这些基因在菌株间的核苷酸序列完全相同，用这些基因和管家基因 efp 和 ihfA 进行的系统发育研究表明，黄单胞菌处于 X. campestris 组和另一种维管束植物病原体 Xylella fastidiosa 之间的进化道路上。黄单胞菌全基因组测序可能是破译黄单胞菌致病性相关分子机制的下一步。
