Phytopathology. 2005 Dec;95(12):1374-80. doi: 10.1094/PHYTO-95-1374.
ABSTRACT The failure to adequately identify plant pathogens from culture-based morphological techniques has led to the development of culture-independent molecular approaches. Increasingly, diagnostic laboratories are pursuing fast routine methods that provide reliable identification, sensitive detection, and accurate quantification of plant pathogens. In addition, since plants or parts thereof can be infected by multiple pathogens, multiplex assays that can detect and quantify different pathogens simultaneously are highly desirable. Technologies that can meet these requirements, especially those involving polymerase chain reaction, are being developed and implemented in horticultural and agricultural practice. Currently, DNA array technology is the most suitable technique for multiplex detection of plant pathogens. Recently, a quantitative aspect was added to this technology, making DNA arrays highly attractive for various research and practical applications. Here, we review the most important recent advances in molecular plant pathogen diagnostics, with special attention to fungal molecular diagnostics. In addition to their applicability in practice, the different criteria that have to be fulfilled for developing robust detection procedures that can routinely be used by diagnostic laboratories are discussed.
摘要 基于培养的形态学技术未能充分识别植物病原体，导致了非培养的分子方法的发展。越来越多的诊断实验室正在寻求快速的常规方法，这些方法能够可靠地识别、敏感地检测和准确地定量植物病原体。此外，由于植物或其部分可能被多种病原体感染，因此同时检测和定量不同病原体的多重分析方法是非常需要的。能够满足这些要求的技术，特别是涉及聚合酶链反应的技术，正在园艺和农业实践中得到开发和实施。目前，DNA 芯片技术是用于植物病原体多重检测的最适合技术。最近，该技术增加了定量方面的内容，使得 DNA 芯片在各种研究和实际应用中极具吸引力。在这里，我们回顾了分子植物病原体诊断方面的最新重要进展，特别关注真菌的分子诊断。除了它们在实践中的适用性之外，还讨论了为开发能够由诊断实验室常规使用的稳健检测程序而必须满足的不同标准。
