Tapchaisri P, Nopparatana C, Chaicumpa W, Setasuban P
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Int J Parasitol. 1991 Jun;21(3):315-9. doi: 10.1016/0020-7519(91)90033-4.
Sera from four patients with parasitologically confirmed gnathostomiasis, 15 patients with presumptive gnathostomiasis, 64 patients with various parasitic infections and 19 healthy adults were studied by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis for their reactivities against somatic extract of Gnathostoma spinigerum third-stage larvae (L3). It was found that the L3 extract was highly complex consisting of more than 20 antigenic components, a few of which gave reactions with sera from the healthy controls. Extensive cross-reactions of the parasite's antigen with sera from patients with other parasitic infections occurred. A specific antigen of G. spinigerum with a mol. wt of 24,000 (24k) was found to react with all parasitologically proven patients, five of the presumptive patients, one of the patients with other parasitic infections and none of the healthy individuals. This 24k component of G. spinigerum is a potential diagnostic antigen for use in the immunodiagnosis of human gnathostomiasis.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹分析，研究了4例经寄生虫学确诊的颚口线虫病患者、15例疑似颚口线虫病患者、64例患有各种寄生虫感染的患者以及19名健康成年人的血清，以检测其对棘颚口线虫三期幼虫（L3）虫体提取物的反应性。结果发现，L3提取物高度复杂，由20多种抗原成分组成，其中一些与健康对照者的血清发生反应。该寄生虫抗原与其他寄生虫感染患者的血清发生了广泛的交叉反应。发现一种分子量为24000（24k）的棘颚口线虫特异性抗原与所有经寄生虫学证实的患者、5例疑似患者、1例其他寄生虫感染患者的血清发生反应，而与所有健康个体的血清均无反应。棘颚口线虫的这种24k成分是用于人类颚口线虫病免疫诊断的潜在诊断抗原。
