Hurtado-Gonzáles O, Aragon-Caballero L, Apaza-Tapia W, Donahoo R, Lamour K
University of Tennessee, Department of Entomology and Plant Pathology, Knoxville 37996, USA.
Phytopathology. 2008 Jun;98(6):688-94. doi: 10.1094/PHYTO-98-6-0688.
Phytophthora capsici is a soilborne pathogen that causes significant losses to pepper production in Peru. Our objective was to investigate the mechanisms by which P. capsici is able to survive and spread. During 2005 to 2007, 227 isolates of P. capsici were collected from four species of pepper (Capsicum annum, C. baccatum, C. chinense, and C. pubescens) and tomato (Solanum lycopersicum) at 33 field sites in 13 provinces across coastal Peru. All 227 isolates were of the A2 mating type and amplified fragment length polymorphism (AFLP) analysis indicates that 221 of the isolates had the same genotype. Analyses of six polymorphic single nucleotide polymorphism (SNP) loci showed fixed heterozygosity suggesting a single clonal lineage is widely dispersed. Members of the same clonal lineage were recovered during 2005 to 2007 from geographically separate locations from each of the host types sampled. Our results indicate that clonal reproduction drives the population structure of P. capsici in Peru. The impact of continuous cropping and irrigation from common river sources on the population structure in Barranca Valley are discussed.
辣椒疫霉是一种土传病原菌，给秘鲁的辣椒生产造成了重大损失。我们的目标是研究辣椒疫霉能够存活和传播的机制。在2005年至2007年期间，从秘鲁沿海13个省份的33个田间地点的四种辣椒（辣椒、黄灯笼辣椒、中国辣椒和毛辣椒）和番茄（番茄）中收集了227株辣椒疫霉分离株。所有227个分离株均为A2交配型，扩增片段长度多态性（AFLP）分析表明，其中221个分离株具有相同的基因型。对六个多态性单核苷酸多态性（SNP）位点的分析显示固定杂合性，表明单一克隆谱系广泛分布。在2005年至2007年期间，从每个采样宿主类型的地理上分开的地点回收了相同克隆谱系的成员。我们的结果表明，克隆繁殖驱动了秘鲁辣椒疫霉的种群结构。讨论了连作和来自共同河流源头的灌溉对巴兰卡山谷种群结构的影响。
