Phytopathology. 2007 Feb;97(2):155-61. doi: 10.1094/PHYTO-97-2-0155.
ABSTRACT Septoria speckled leaf blotch (SSLB) caused by Septoria passerinii is a common disease in barley. SSLB resistance genes Rsp1, Rsp2, and Rsp3 have previously been identified in the United States Department of Agriculture National Small Grains collection accessions CIho 14300, CIho 4780, and CIho 10644, respectively. Populations of 100 to 120 F(2) individuals were evaluated for SSLB resistance in the greenhouse. Inheritance was evaluated in F(2:3)-derived families in the field. Partial molecular maps for three Rsp genes were constructed on F(2) and F(2:3) families derived from crosses between Robust and the resistant accessions CIho 14300, CIho 4780, and CIho 10644. The resistant locus Rsp1 was mapped to the short arm of chromosome 3H with two flanking diversity arrays technology (DArT) markers, bPb-6978 (8.9 cM) and bPb-9945 (16.3 cM), and two random amplified polymorphic DNA (RAPD) markers, OPC2(441R) (3.0 cM) and UBC285(158R) (4.3 cM). The genes Rsp2 and Rsp3 were positioned on the short arm of barley chromosome 1H with two restriction fragment length polymorphism (RFLP), six DArT, and three RAPD markers. An RFLP marker, MWG938, and an RAPD marker, OPAH5(545C), were tightly associated with Rsp2 at a distance of 0 cM. Five DArT markers spanning the short arm of 1H surrounded Rsp3 at a distance of 2.3 and 5.8 cM, while two RAPD markers-OPBA12(314C) (2.4 cM) in coupling and OPB17(451R) (3.5 cM) in repulsion-flanked Rsp3. Molecular marker data associated with Rsp2 and Rsp3 indicated that the two genes are closely linked on chromosome 1HS. A total of 17 of 154 simple sequence repeats (SSRs) tested were associated with Rsp genes on chromosome 1H and 3H, and they were also integrated into genetic linkage maps of the three F(2) Robust populations. Knowledge about the map position of Rsp genes on barley chromosomes will be useful for breeding for SSLB resistance in barley and eventual gene cloning.
摘要 由叶点霉菌引起的叶斑病是大麦的一种常见病。美国农业部国家小谷物收藏品系 CIho 14300、CIho 4780 和 CIho 10644 中分别鉴定出了 Rsp1、Rsp2 和 Rsp3 这三个抗叶斑病基因。在温室中，对 100 到 120 个 F2 个体进行了叶斑病抗性评价。在田间 F2:3 衍生的家系中进行了遗传评估。从 Robust 与抗性品系 CIho 14300、CIho 4780 和 CIho 10644 之间的杂交后代中构建了三个 Rsp 基因的部分分子图谱。抗病基因 Rsp1 被定位在 3H 染色体的短臂上，两个侧翼的多样性数组技术（DArT）标记 bPb-6978（8.9 cM）和 bPb-9945（16.3 cM），以及两个随机扩增多态性 DNA（RAPD）标记 OPC2(441R)（3.0 cM）和 UBC285(158R)（4.3 cM）。Rsp2 和 Rsp3 被定位在大麦 1H 染色体的短臂上，与两个限制片段长度多态性（RFLP）、六个 DArT 和三个 RAPD 标记相关。一个 RFLP 标记 MWG938 和一个 RAPD 标记 OPAH5(545C)与 Rsp2 紧密相关，距离为 0 cM。五个 DArT 标记跨越 1H 的短臂，距离 Rsp3 为 2.3 和 5.8 cM，而两个 RAPD 标记 OPAH5(545C)（2.4 cM）在连锁和 OPAH5(545C)（3.5 cM）在排斥状态下与 Rsp3 紧密相连。与 Rsp2 和 Rsp3 相关的分子标记数据表明，这两个基因在 1HS 染色体上紧密连锁。在 154 个简单序列重复（SSR）中，有 17 个与 1H 和 3H 染色体上的 Rsp 基因相关，它们也被整合到三个 Robust F2 群体的遗传连锁图谱中。关于 Rsp 基因在大麦染色体上的图谱位置的知识将有助于大麦叶斑病抗性的选育和最终基因克隆。
