Golegaonkar Prashant G, Karaoglu Haydar, Park Robert F
Plant Breeding Institute Cobbitty, University of Sydney, Private Mail Bag 11, Camden, NSW 2570, Australia.
Theor Appl Genet. 2009 Nov;119(7):1281-8. doi: 10.1007/s00122-009-1132-0. Epub 2009 Aug 27.
An incompletely dominant gene conferring resistance to Puccinia hordei, Rph14, identified previously in an accession of Hordeum vulgare, confers resistance to all known pathotypes of P. hordei in Australia. Knowledge of the chromosomal location of Rph14 and the identification of DNA markers closely linked to it will facilitate combining it with other important leaf rust resistance genes to achieve long lasting resistance. The inheritance of Rph14 was confirmed using 146 and 106 F(3) lines derived from the crosses 'Baudin'/'PI 584760' (Rph14) and 'Ricardo'/'PI 584760' (Rph14), respectively. Bulk segregant analysis on DNA from the parental genotypes and resistant and susceptible DNA bulks using DArT markers located Rph14 to the short arm of chromosome 2H. DArT marker bPb-1664 was identified as having the closest genetic association with Rph14. PCR based marker analysis identified a single SSR marker, Bmag692, linked closely to Rph14 at a map distance of 2.1 and 3.8 cm in the 'Baudin'/'PI 584760'and 'Ricardo'/'PI 584760' populations, respectively.
先前在一个大麦品种中鉴定出的一个对大麦柄锈菌具有抗性的不完全显性基因Rph14，对澳大利亚所有已知的大麦柄锈菌致病型都具有抗性。了解Rph14的染色体定位以及与其紧密连锁的DNA标记，将有助于把它与其他重要的叶锈病抗性基因结合起来，以实现持久抗性。分别利用来自杂交组合“Baudin”/“PI 584760”（Rph14）和“Ricardo”/“PI 584760”（Rph14）的146个和106个F(3)株系，证实了Rph14的遗传特性。利用DArT标记对亲本基因型以及抗性和感病DNA混合池的DNA进行分离群体分析法，将Rph14定位到2H染色体的短臂上。DArT标记bPb - 1664被确定与Rph14具有最紧密的遗传关联。基于PCR的标记分析确定了一个单一的SSR标记Bmag692，在“Baudin”/“PI 584760”和“Ricardo”/“PI 58476 population群体中，它与Rph14紧密连锁，遗传距离分别为2.1和3.8厘摩。
