Phytopathology. 2007 Feb;97(2):170-8. doi: 10.1094/PHYTO-97-2-0170.
ABSTRACT Magnaporthe grisea (anamorph = Pyricularia grisea) causes blast on rice (Oryza sativa) and gray leaf spot on turfgrass. Gray leaf spot is a serious disease on St. Augustinegrass (Stenotaphrum secundatum), perennial ryegrass (Lolium perenne), and tall fescue (Festuca arundinacea). Virulence assays performed in this study revealed that M. grisea collected from rice could also cause disease on St. Augustinegrass and tall fescue. One rice isolate, Che86061, caused similar disease reactions on susceptible cultivars of rice and St. Augustinegrass and an incompatible interaction on resistant cultivars of both species. To explore whether similar defense-related genes are expressed in rice and St. Augustinegrass, a rice cDNA library was screened using pooled cDNAs derived from M. grisea-infected St. Augustinegrass. Thirty rice EST (expressed sequence tag) clones showing differential expression in St. Augustinegrass following M. grisea inoculation were identified and classified into six putative functional groups. Northern blot analyses of seven EST clones that collectively represented each putative functional group confirmed that the expression of five out of seven EST clones was similar in both rice and St. Augustinegrass. This study represents one of the first attempts to use a broad-scale genomic approach and resources of a model monocot system to study defense gene expression in St. Augustinegrass following M. grisea infection.
摘要 稻瘟病菌（无性型 = 灰梨孢）会引起水稻稻瘟病和草坪草灰斑病。灰斑病是圣奥古斯丁草（钝叶草）、多年生黑麦草和高羊茅的严重病害。本研究中的致病性测定表明，从水稻上采集的稻瘟病菌也能引起圣奥古斯丁草和高羊茅发病。一个水稻分离株 Che86061，在水稻和圣奥古斯丁草的感病品种上引起相似的病害反应，而在两个物种的抗病品种上则表现出不亲和的互作。为了探索在水稻和圣奥古斯丁草中是否表达了相似的防御相关基因，我们使用从感染了稻瘟病菌的圣奥古斯丁草中提取的混合 cDNA 筛选了水稻 cDNA 文库。鉴定出 30 个在感染稻瘟病菌后在圣奥古斯丁草中差异表达的水稻 EST（表达序列标签）克隆，并将其分为六个假定的功能组。对代表每个假定功能组的七个 EST 克隆的 Northern blot 分析证实，七个 EST 克隆中有五个在水稻和圣奥古斯丁草中的表达相似。本研究是首次利用广泛的基因组方法和模式单子叶系统资源来研究稻瘟病菌感染圣奥古斯丁草后的防御基因表达的研究之一。
