Phytopathology. 2000 Oct;90(10):1079-88. doi: 10.1094/PHYTO.2000.90.10.1079.
ABSTRACT Fusarium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat and barley. Barley grains infected with deoxynivalenol (DON), a vomitoxin produced by Fusarium graminearum, are rejected for malting and brewing. Among six-rowed barley cultivars tested thus far, only cv. Chevron exhibited resistance. This study was conducted to map genes and to identify DNA markers for marker-assisted breeding for FHB resistance in cv. Chevron with restriction fragment length polymorphism (RFLP) markers. A doubled haploid (DH) population was created from a cross between cv. Chevron and susceptible cv. Stander. Seven field experiments were conducted in four different locations in 2 years. A RFLP map containing 211 loci and covering over 1,000 centimorgans (cM) of the genome was used to map quantitative trait loci (QTL) associated with relatively low FHB severity and DON concentration. Morphological traits differing between the parents were also measured: heading date, plant height, spike angle, number of nodes per cm of rachis in the spike, and kernel plumpness. Many of the QTL for FHB and DON coincided with QTLs for these morphological traits. The "fix-QTL" algorithm in Mapmaker QTL was used to remove the part of the variance for FHB resistance that may be explained by heading date or plant height. Results from this study suggest that QTLs with major effects for FHB resistance probably do not exist in cv. Chevron. Three QTL intervals, Xcmwg706-Xbcd441 on chromosome 1H, Xbcd307b-Xcdo684b on chromosome 2H, and Xcdo959b-Xabg472 on chromosome 4H, that are not associated with late heading or height may be useful for marker-assisted selection.
摘要 镰刀菌穗腐病（FHB）是一种毁灭性疾病，可导致小麦和大麦产量和质量显著下降。感染禾谷镰刀菌产生的呕吐毒素（DON）的大麦粒被拒绝用于麦芽和酿造。在迄今为止测试的六棱大麦品种中，只有 Chevron 品种表现出抗性。本研究旨在利用限制片段长度多态性（RFLP）标记，对 Chevron 品种进行基因图谱绘制和用于 FHB 抗性的 DNA 标记鉴定，以进行标记辅助选择。 Chevron 和易感品种 Stander 杂交产生了双单倍体（DH）群体。在 2 年的 4 个不同地点进行了 7 个田间试验。一个包含 211 个位点、覆盖基因组 1000 多厘摩（cM）的 RFLP 图谱被用于定位与相对低的 FHB 严重程度和 DON 浓度相关的数量性状位点（QTL）。还测量了父母本之间不同的形态特征：抽穗期、株高、穗角、穗轴每厘米节数和籽粒饱满度。许多与 FHB 和 DON 相关的 QTL 与这些形态特征的 QTL 重叠。Mapmaker QTL 中的“fix-QTL”算法用于去除 FHB 抗性的部分方差，该方差可能由抽穗期或株高来解释。本研究结果表明，Chevron 品种中可能不存在对 FHB 抗性有重大影响的 QTL。三个 QTL 区间，1H 染色体上的 Xcmwg706-Xbcd441、2H 染色体上的 Xbcd307b-Xcdo684b 和 4H 染色体上的 Xcdo959b-Xabg472，与晚抽穗或高无关，可能对标记辅助选择有用。
