de la Pena R C, Smith K P, Capettini F, Muehlbauer G J, Gallo-Meagher M, Dill-Macky R, Somers D A, Rasmusson D C
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, 55108, XX.
Theor Appl Genet. 1999 Aug;99(3-4):561-9. doi: 10.1007/s001220051269.
Resistance to Fusarium head blight (FHB), deoxynivalenol (DON) accumulation, and kernel discoloration (KD) in barley are difficult traits to introgress into elite varieties because current screening methods are laborious and disease levels are strongly influenced by environment. To improve breeding strategies directed toward enhancing these traits, we identified genomic regions containing quantitative trait loci (QTLs) associated with resistance to FHB, DON accumulation, and KD in a breeding population of F(4:7) lines using restriction fragment length polymorphic (RFLP) markers. We evaluated 101 F(4:7) lines, derived from a cross between the cultivar Chevron and an elite breeding line, M69, for each of the traits in three or four environments. We used 94 previously mapped RFLP markers to create a linkage map. Using composite interval mapping, we identified 10, 11, and 4 QTLs associated with resistance to FHB, DON accumulation, and KD, respectively. Markers flanking these QTLs should be useful for introgressing resistance to FHB, DON accumulation, and KD into elite barley cultivars.
在大麦中，对赤霉病（FHB）的抗性、脱氧雪腐镰刀菌烯醇（DON）的积累以及籽粒变色（KD）是难以导入优良品种的性状，因为目前的筛选方法费力，且病害水平受环境影响很大。为了改进旨在增强这些性状的育种策略，我们使用限制性片段长度多态性（RFLP）标记，在一个F(4:7)株系的育种群体中，鉴定出了包含与FHB抗性、DON积累和KD相关的数量性状位点（QTL）的基因组区域。我们在三到四个环境中，针对每个性状评估了101个由品种Chevron和一个优良育种系M69杂交产生的F(4:7)株系。我们使用94个先前定位的RFLP标记创建了一个连锁图谱。通过复合区间作图，我们分别鉴定出了10个、11个和4个与FHB抗性、DON积累和KD相关的QTL。这些QTL侧翼的标记对于将FHB抗性、DON积累和KD导入优良大麦品种应该是有用的。
